结合SSH和cDNA芯片技术在植物研究中的应用  

Application of SSH and cDNA chip in plant

作　　者：周忠静[1] 陈珍补[1] 洛雪[2] 

机构地区：[1]浙江大学农业与生物技术学院农学系,浙江杭州310029 [2]黑龙江大学生命科学学院,哈尔滨150080

出　　处：《生物信息学》2005年第4期181-184,共4页Chinese Journal of Bioinformatics

摘　　要：抑制性差减杂交(Suppression Subtractive Hybridization,SSH)技术是分离差异表达基因的一种新方法。cDNA芯片也是近年来发展起来的一种新技术,它是指将大量的特定的寡核苷酸片段或基因片段作为探针,有规律地排列固定于硅片、玻片、塑料片等固相支持物上制成的芯片。本文主要介绍抑制差减杂交和cDNA芯片技术原理及其在植物研究中的应用。Suppreesion Subtractive Hybfidizafion,SSH is a new method for cloning differentially expressed genes, eDNA chip can be fabricated on solid - phase carriers by light - directed in situ syntheses and mieroprinting . On this chip , many DNA probes are aligned orderly . After hybridization of the gene chip with the labeled sample targets, scanning the DNA microarray and the computer analysis can retrieve highthroughput gene sequence and expression information .In plant ,combining SSH and eDNA chip can been used for discovery of correlated genes and new gene analysis of gene expression , detection of gene mutation and polymorphism and DNA sequencing etc.

关 键 词：抑制差减杂炙 CDNA芯片 植物 应用 

分 类 号：Q753[生物学—分子生物学] Q943