Sequence-specific Hydrolysis of Single-stranded DNA by PNA-Cerium （Ⅳ） Adduct  

Sequence-specific Hydrolysis of Single-stranded DNA by PNA-Cerium (IV) Adduct

作　　者：He Bai SHEN Feng WANG Yong Tao YANG 

机构地区：[1]College of Life ＆ Environment Science, Shanghai Normal University, Shanghai 200234

出　　处：《Chinese Chemical Letters》2005年第12期1663-1666,共4页中国化学快报（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(No.20443005);the Nanotechnology Special Projects of Shanghai(No.0352nm123)

摘　　要：A novel artificial site specific cleavage reagent, with peptide nucleic acid （PNA） as sequence-recognizing moiety and cerium （IV） ions as “scissors” for cleaving target DNA, was synthesized. Subsequently, it was employed in the cleavage of target 26-mer single-stranded DNA （ssDNA）, which has 10-mer sequence complementary with PNA recognizer in the hybrids, under physiological conditions. Reversed-phase high-performance liquid chromatogram （RPHPLC） experiments indicated that the artificial site specific cleavage reagent could cleave the target DNA specifically.A novel artificial site specific cleavage reagent, with peptide nucleic acid （PNA） as sequence-recognizing moiety and cerium （IV） ions as “scissors” for cleaving target DNA, was synthesized. Subsequently, it was employed in the cleavage of target 26-mer single-stranded DNA （ssDNA）, which has 10-mer sequence complementary with PNA recognizer in the hybrids, under physiological conditions. Reversed-phase high-performance liquid chromatogram （RPHPLC） experiments indicated that the artificial site specific cleavage reagent could cleave the target DNA specifically.

关 键 词：Peptide nucleic acid （PNA） artificial site specific cleavage reagent HYDROLYSIS reversed-phase high-performance liquid chromatogram （RP-HPLC） 

分 类 号：O614.332[理学—无机化学]