死胎肾脏组织中HBVDNA表达的临床和病理研究  

The Clinical and Pathological Study on the Expression of HBVDNA in the Kidney Tissues of the Dead Fetus

作　　者：陈宏[1] 赵伟[2] 

机构地区：[1]南京大学医学院附属鼓楼医院妇产科,210008 [2]南京东南大学医学院附属南京市第二医院

出　　处：《中国全科医学》2005年第24期2025-2026,共2页Chinese General Practice

基　　金：铁道部2000年自然科技资金资助(J2000Z095)

摘　　要：目的观察死胎肾脏组织中HBVDNA的表达情况,探讨通过产妇传播到死胎肾脏组织中的HBV是否存在复制。方法采集40例乙型肝炎产妇产下的死胎,常规尸检,取肾脏组织。采用原位分子杂交法,检测其中的HBVDNA;收集产妇产前静脉血HBV的检测结果。结果死胎肾脏组织中HBVDNA的检出率为65·0%(26/40)。产妇产前静脉血HBV呈高、低和无复制状态时,肾脏组织中HBVDNA的检出率分别为2/4、50·0%(10/20)例和88·0%(14/16),差别有显著性意义(P<0·05)。HBVDNA颗粒在肾实质细胞(近、远曲小管上皮细胞和肾血管球毛细血管内皮细胞,球内、外系膜细胞,球旁细胞,足细胞)浆,肾脏血管中呈点、灶状分布,少数在肾实质细胞核上。结论死胎肾脏组织中有HBV复制;死胎肾脏组织中HBVDNA表达与产妇血清中HBV复制状态有关。Objective To observe the expression of HBVDNA and study if there was copy of HBV in the kidney tissues of the dead fetus that the puerperal delivery with hepatitis virus B. Methods 40 cases with dead fetus collected were made about the autopsy for kidney tissues and HBVDNA detected with in situ molecule hybridization technique. The detect result was followed up on HBV in the mother veins before delivery. Results For the kidney tissues, there were 26 （ 65.0% ） cases detected out HBVDNA. When the HBV was high, low and without replication status, it was2/4 （50. 0% ） cases, 10/20 （50. 0% ） cases and 14/16 （88.0%） cases for the rate of HBVDNA in the kidney tissues. For the rate of HBVDNA, it was statistically significant for the difference among the high and low, without replication status （ P 〈 0.05 ）. HBVDNA was mainly in the kidney cells and the kidney vessels, and fewness in the kidney cells nuclei. Conclusion There were HBV replication in the kidney tissues of the dead fetus. The expression of HBVDNA in the kidney tissues of the dead fetus was related with HBV replication status in the pregnant woman veins.

关 键 词：乙型肝炎病毒脱氧核糖核酸 死胎 肾脏组织 原位分子杂交 

分 类 号：R512.62[医药卫生—内科学]