毫米波辐射孕鼠对子鼠海马区c-Fos蛋白表达与学习记忆的相关研究  

Relationship between millimeter wave irradiation in pregnant mice and c-Fos protein expression in hippocampus and learning and memory functions in their offsprings

作　　者：赵志刚[1] 张劲风[2] 阎平[3] 马艳梅[1] 

机构地区：[1]解放军210医院传染科,大连116021 [2]解放军210医院病理科,大连116021 [3]大连医科大学中心实验室

出　　处：《中华劳动卫生职业病杂志》2005年第6期424-427,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目(39600034);辽宁省自然科学基金资助项目(20032135)

摘　　要：目的确定毫米波导致胚胎损伤的阈值,探讨毫米波导致子鼠学习记忆功能降低的机制及毫米波是否具有非热效应.方法用37.4、42.2、53.0、60.0 GHz,功率密度1、3、5、8 mW/cm2毫米波,在小鼠怀孕6～15 d时每天进行2 h辐射,用Y型电迷宫对子鼠进行学习记忆功能测试,在子鼠学习训练后的0、30、60、90、120min,用免疫组化方法对其海马进行c-Fos蛋白表达水平的定量分析.结果37.4、42.2 GHz和53.0、60.0 GHz毫米波导致子鼠学习记忆功能降低,海马区c-F0s蛋白表达水平降低的最小功率密度分别为5和3 mW/cm2.随功率密度的增加,辐射导致子鼠学习记忆功能损伤程度加重.毫米波未使孕鼠体温升高.结论37.4、42.2 GHz和53.0、60.0 GHz毫米波导致子鼠损伤的阈值分别为5、3 mW/cm2,毫米波导致子鼠学习记忆功能降低与其海马区c-Fos蛋白表达水平受抑制相关,毫米波辐射具有非热效应.Objective To determine the threshold of millimeter wave irradiation for fetal injury in mice and the mechanism of decrease of learning and memory function in their offsprings and to verify whether the millimeter wave has the non-thermal effect. Methods Pregnant mice were irradiated by millimeter wave with frequencies of 37.4,42.2,53.0 and 60.0 GHz at power densities of 1,3,5,8 mW/cm^2 for two hours daily from the 6th to 15th day of their gestation. Learning and memory functions of their offsprings were tested by a Y-type electric maze. c-Fos protein expression level in hippocampus of their offsprings was determined with immunohistochemistry 0,30,60,90 and 120 minutes after the offsprings were trained respectively. Results The minimal power density of millimeter wave for the decrease in learning and memory function and decrease of c-Fos protein expression level in hippocampus of their offsprings caused by 37.4,42.2 GHz and 53.0,60.0 GHz was 5 and 3 mW/cm^2. Severity of injury for learning and memory in offsprings caused by irradiation increased with the power density of millimeter wave. The millimeter wave did not cause increase of the body temperature of the pregnant mice. Conclusion The threshold of millimeter wave with 37.4,42.2 GHz, and 53.0,60.0 GHz causing fetal injury in mice is 5 and 3 mW/cm2 respectively. The decrease in learning and memory functions in offspring mice is related with decrease of c-Fos protein expression level in hippocampus. Millimeter wave has the non-thermal effects.

关 键 词：微波 辐射损伤 实验性 原癌基因蛋白质C-FOS类 胚胎 

分 类 号：Q691[生物学—生物物理学]