双歧杆菌DNA对小鼠肠上皮内淋巴细胞产生γ干扰素和白介素2作用的研究  

Effect of bifidobacteria DNA on the production of interferon-γ and interleukin-2 in intestinal intraepithelial lymphocytes of mice

作　　者：王舰[1] 史俊岩[1] 罗恩杰[1] 周正任[1] 

机构地区：[1]中国医科大学基础医学院病原生物学教研室,辽宁沈阳110001

出　　处：《中国医科大学学报》2005年第6期523-524,共2页Journal of China Medical University

摘　　要：目的:研究双歧杆菌DNA对小鼠肠上皮内淋巴细胞(iIEL)γ干扰素(IFN-γ)和白介素2(IL-2)产生水平的影响,探讨其对小鼠iIEL免疫激活作用。方法:提取双歧杆菌DNA,肌肉注射免疫小鼠,提取小鼠iIEL,以IFNγ和IL-2作为检测指标,测定其变化。结果:DNA免疫小鼠产生IFN-γ和IL-2的水平与对照组相比明显提高(P<0.01)。结论:双歧杆菌DNA明显提高小鼠iIEL产生IFN-γ和IL-2的能力。Objective ： To study the effect of bifidobacteria DNA on the production of interferon - γ, （ IFN - γ） and interleukin-2 （IL-2） in intestinal intraepithelial lymphocytes （iIEL） of mice, and to discuss the effect of immune activation of bifidobacteria DNA. Methods：The bifidobacteria DNA was extracted and injected muscularly into immune mice. The iIEL was isolated, and the levels of IFN-γ, and IL-2 were determined. Results：The levels of IFN-γ, and IL-2 in DNA-immunized mice were higher than those in controls （ P 〈 0.01）. Conclusion：Bifidobacteria DNA can greatly improve the production of IFN-γand IL-2 in iIEL of mice.

关 键 词：双歧杆菌DNA Γ干扰素 白介素2 肠上皮内淋巴细胞 

分 类 号：R392.12[医药卫生—免疫学]