新生物活性肽—daintain/AIF-1在乳腺癌中的表达  

A new active peptide-daintain/AIF-1's expression in breast carcinomas

作　　者：李发芳[1] 陈正望[1] 

机构地区：[1]华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《湖北大学学报（自然科学版）》2005年第4期376-380,共5页Journal of Hubei University：Natural Science

基　　金：国家高新技术研究发展863计划(No.2002AA214061);国家自然科学基金(No.30370647;No30470823)资助项目

摘　　要：采用免疫组织化学两步法分析了乳腺癌组织蜡块切片中daintain/AIF-1的表达.93%的乳腺癌中都有该活性肽的分布,癌旁良性组织中则没有阳性染色或染色很浅.Daintain/AIF-1在乳腺病变级别不同组织中的表达差异很大:增生组织免疫后着黄色,重度不典型增生组织着橙黄色,癌组织则染成了棕褐色.进一步用RT-PCR检测发现,乳腺癌新鲜组织中能扩增出daintain/AIF-1的mRNA,而癌旁良性乳腺组织中的daintain/AIF-1 mRNA极微量,几乎看不到扩增的核酸条带.这些研究表明,daintain/AIF-1在乳腺癌中过量表达,可能在乳腺肿瘤的发展过程起到了促进作用.因此,提示daintain/AIF-1可作为乳腺癌病变早期诊断的一个参考指标.Immunochemistry is applied to analysis the distribution of daintain/AIF - 1 in breast tissues. Significantly higher positive immunostaining rate （93 % ） of daintain was visible than those identified biomarkers in breast cancer cases, such as BAG - 1 （65 % ） etc. No staining or weak staining was seen in benign tissues beside the carcinomas. The distribution of daintain/AIF- 1 among benign cells,hyperplasia tissues, immature hyperplasia and malignant tumor was apparently distinct, and it presented the progression to high malignant lesion. In parallel, by using RT - PCR it was also found daintain/AIF - 1 mRNA in fresh freezing breast tissues. The result showed that there were daintain/AIF - 1 mRNA presenting in breast cancers,but no smear or small smear of daintain expressing in benign breast tissues. Over expression of daintain/AIF - 1 in breast tissues came mainly from cancers. It is suggest that detecting for daintain/AIF - 1 potentially may be useful for determination in an early diagnosis and for monitoring prognosis of patients undergone treatments.

关 键 词：乳腺癌 daintain/AIF-1 免疫组织化学 反转录-聚合酶链式反应 

分 类 号：Q51[生物学—生物化学]