Virus-induced silencing of a tobacco deoxyhypusine synthase gene  

作　　者：WANG Hongzhi MA Rongcai LI Ruifen WANG Guoying WEI Jianhua 

机构地区：[1]State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijng 100094, China [2]Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100089, China

出　　处：《Chinese Science Bulletin》2005年第23期2707-2713,共7页

基　　金：This work was supported by the Natural Science Foundation of Beijing(Grant No.5032008).

摘　　要：A cDNA fragment corresponding to deoxyhy-pusine synthase gene NbDHS was isolated and cloned into potato virus X (PVX) vector for functional analysis in Nico-tiana benthamiana by using virus-induced gene silencing (VIGS). Plants agroinfected with recombinant virus vector PVX-NbDHS exhibited an increase in leaf biomass, delay in natural leaf senescence and flowering time, and decrease in leaf chlorophyll content. Semi-quantitative RT-PCR and Northern analysis showed that the transcript level of DHS was significantly lower in PVX-NbDHS infected plants. At the same time, the expression for eIF-5A, the target proteins of DHS in N. benthamiana, was concomitantly suppressed by semi-quantitative RT-PCR and Western analysis. From the phenotypic feature of the infected plants and the reduced expression abundance of DHS and eIF-5A, we concluded that NbDHS plays important roles in plant growth, development and senescence. The possible application of DHS gene in ge-netic modification of crops and horticultural plants was dis-cussed.A cDNA fragment corresponding to deoxyhypusine synthase gene NbDHS was isolated and cloned into potato virus X （PVX） vector for functional analysis in Nico. tiana benthamiana by using virus-induced gene silencing （VIGS）. Plants agroinfected with recombinant virus vector PVX-NbDHS exhibited an increase in leaf biomass, delay in natural leaf senescence and flowering time, and decrease in leaf chlorophyll content. Semi-quantitative RT-PCR and Northern analysis showed that the transcript level of DHS was significantly lower in PVX-NbDHS infected plants. At the same time, the expression for eIF-5A, the target proteins of DHS in N. benthamiana, was concomitantly suppressed by semi-quantitative RT-PCR and Western analysis. From the phenotypic feature of the infected plants and the reduced expression abundance of DHS and eIF-5A, we concluded that NbDHS plays important roles in plant growth, development and senescence. The possible application of DHS gene in genetic modification of crops and horticultural plants was discussed.

关 键 词：烟草 脱氧羟腐胺缩赖氨酸合酶基因 基因克隆 生长促进作用 

分 类 号：Q78[生物学—分子生物学]