Construction and characteristics of a transformed lepidopteran cell clone expressing baculovirus p35  

作　　者：ZHENG Gulling LI Changyou LI Guoxun WANG Ping Robert R. Granados 

机构地区：[1]Department of Plant Protection, Laiyang Acricultural College, Qingdao, 266109 [2]Boyce Thompson Institute at Cornell University, Ithaca NY 14850, USA

出　　处：《Chinese Science Bulletin》2005年第23期2728-2732,共5页

基　　金：This work was supported in part by the National Natural Science Foundation of China(Grant No.30370963);Laiyang Agricultural College Foundation(Grant No.630320).

摘　　要：A transformed cell line was constructed fromMythimna separata cells Ms7311 by lipofection methodTMs7311 cells were generated using a double selection tech-nique involving a selection in the antibiotic Zeocin, followedby a second round of selection by exhibiting cell characteri-zation. A cell clone expressing p35 was obtained with highlevel of AcMNPV and recombinant proteins. Compared withwild type Ms7311 cells, the cell clone showed increased resis-tance to Actinamycin D-induced apoptosis and a profoundresistance to nutrient development (PBS). When the cellclone was infected with recombinant baculoviruses express-ing secreted alkaline phosphatase (SEAP) and β-galactosi-dase, expression of the recombinant proteins from TMs7311cells exceeded that from parental Ms7311 cells. Production ofbudded virus and occlusion body was significantly higherthan that from parental cells Ms7311.A transformed cell line was constructed from Mythimna separata cells Ms7311 by lipofection method. TMs7311 cells were generated using a double selection technique involving a selection in the antibiotic Zeocin, followed by a second round of selection by exhibiting cell characterization. A cell clone expressing p35 was obtained with high level of AcMNPV and recombinant proteins. Compared with wild type Ms7311 cells, the cell clone showed increased resistance to Actinamycin D-induced apoptosis and a profound resistance to nutrient development （PBS）. When the cell clone was infected with recombinant baculoviruses expressing secreted alkaline phosphatase （SEAP） and β-galactosidase, expression of the recombinant proteins from TMs7311 cells exceeded that from parental Ms7311 cells. Production of budded virus and occlusion body was significantly higher than that from parental ceils Ms7311.

关 键 词：鳞翅目 P35基因 蛋白质重组 细胞凋亡 杆状病毒 细胞克隆 

分 类 号：Q969.42[生物学—昆虫学] Q78