转Pti5-VP16基因小麦抗白粉病鉴定及可溶性蛋白分析  

Identification of the disease resistance of transgenic wheat with Pti5-VP16 gene and the analysis of soluble protein

作　　者：魏松红[1] 曹远银[1] 纪明山[1] 

机构地区：[1]沈阳农业大学植物保护学院,辽宁沈阳110161

出　　处：《植物保护》2005年第6期26-28,共3页Plant Protection

基　　金：辽宁省博士启动基金课题(20021039);国家"十五"重点科技攻关项目专题(2001BA509B03-3)

摘　　要：对PCR呈阳性的转基因小麦植株(T0)及T1代植株接种小麦白粉病菌,鉴定结果表明:T0代接种7 d,病害表型多数为抗病;接种14 d,病害表型为抗病、中抗及中感,病害严重度明显轻于对照。T1代接种7 d后,多数表现为中抗、抗,少数为中感;接种28 d后,仅有2株仍表现抗病,其余表现为感病,但病害严重度轻于对照。对T1代植株可溶性蛋白分析表明T1代植株与对照相比多一条新谱带。The transgenic wheat with positive PCR results and the plants of the T1 generation were inoculated with Blumeria graminis. The results showed that the reaction type of To was resistant （R） in the early 7 days after inoculation, but became R, MR or MS 14 days after inoculation. Their disease severity was clearly lighter than that of the controls. Most plants of the T1 generation were MR or R, with a few as MS, 7 days after inoculation. Only 2 plants were R, while others were S, in 28 days after inoculation. Their disease severity was also lighter than that of the controls. Analysis of the soluble proteins of the T1 generation indicated that T1 plants had a new band that was missing in the controls.

关 键 词：植物病理学 转基因小麦 小麦白粉病 抗病性鉴定 

分 类 号：S435.121.46[农业科学—农业昆虫与害虫防治]