重组β-防御素-2对呼吸道绿脓杆菌感染大鼠急性肺损伤的保护作用  

Protective effect of recombioant β-definsin-2 on the lung against acute injury induced by pseudomonas aeruginosa infection in rats

作　　者：王海宏[1] 舒强[2] 石卓[2] 赵正言[2] 方向明[1] 

机构地区：[1]浙江大学邵逸夫临床医学研究所,杭州市310016 [2]浙江大学儿科研究所,杭州市310016

出　　处：《中华麻醉学杂志》2005年第10期762-764,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金资助项目(30070854)

摘　　要：目的 观察重组β-防御素-2对呼吸道绿脓杆菌感染大鼠急性肺损伤(ALI)的保护作用。 方法10只清洁级雄性成年SD大鼠,随机分为防御素组和对照组,每组5只。防御素组大鼠暴露声 门后,气管内滴注5×107PFU／ml重组腺病毒(含有β-防御素-2编码基因)50μl,对照组给予等量对照腺 病毒(不含β-防御素-2编码基因)。48 h后两组气管内滴注6×108CFU／ml绿脓杆菌ATCC27853 200μl, 制备绿脓杆菌感染致ALI模型。气管内滴注绿脓杆菌24 h后处死大鼠,采集肺泡灌洗液,进行绿脓杆 菌菌落数和白细胞计数;观察肺组织病理学变化,并测定肺组织细胞间粘附分子-1(ICAM-1)表达水 平。结果与对照组比较,防御素组BALF中绿脓杆菌菌落数、白细胞计数、肺组织ICAM-1表达水平 及肺病理组织学评分降低(P<0．05)。结论重组β-防御素-2对呼吸道绿脓杆菌感染大鼠ALI有一 定的保护作用,可能与其杀菌作用和下调肺组织ICAM-1的表达有关。Objective The purpose of this study was to determine if recombinant β-defensin-2 can prevent the lung from being injured by pseudomonas aeruginosa infection. Methods Ten male SD rats （class SPF） weighing 250-300 g were randomly divided into 2 groups （ n = 5 each） ： Ⅰ defensin group and Ⅱ control group. The animals were anesthetized with intraperitoneal thiopental 10 mg· kg^- 1 and intubated. 50 μl of 5 × 10^7 PFU·ml^-1 adenovirus with or without β-defensin-2 gene was instilled into the trachea via tracheal tube. 48 h later 200 μl of 6 × 10^8 CFU·ml^-1 pseudomonas aeruginosa ATCC27853 was instilled into the trachea in both groups. All the rats were killed after 24 h and the lungs were removed for （1） histologic examination, （2） determination of ICAM-1 expression in the lung and （3） broncho-alveolar lavage. The number of WBC and pseudomonas aeruginosa in broncho-alveolar lavage fluid （BALF） was counted. Results The number of WBC and pseudomonas aeruginosa in BALF was significantly less in defensin group than in control group. The degree of instologic damage and the expression of ICAM-1 in lung tissue were significantly decreased in the defensin group as compared with control group. Conclusion Recombinant β-defensin-2 can kill pseudomonas aeruginosa in vivo and modulate the expression of ICAM-1. It can protect the lung against injury induced by pseudomonas aeruginosa infection.

关 键 词：防御素类 呼吸窘迫综合征 成人 假单胞菌感染 

分 类 号：R96[医药卫生—药理学]