浊度法测定两性霉素B及其脂质体的效价  

Content determination of amphotericin B and liposomal amphotericin B by turbidimetric method

作　　者：朱会琴[1] 逄涣欢[1] 胡昌勤[1] 冯芳[2] 

机构地区：[1]中国药品生物制品检定所 [2]中国药科大学,南京210009

出　　处：《中国抗生素杂志》2005年第12期752-755,共4页Chinese Journal of Antibiotics

摘　　要：目的建立浊度法测定两性霉素B及其脂质体效价的方法。方法啤酒酵母为实验菌,加菌量2%～3.3%(V/V),(37±1)℃培养6～8h测定。结果抗生素线性浓度为0.04～0.10μg/ml,一剂量法、二剂量法的平均回收率分别为103.5%(n=5)、106.6%(n=2),日内RSD分别为1.64%、0.38%,日间RSD分别为2.79%(n=8)、0.87%(n=3)。结论本方法灵敏,快速,可作为测定两性霉素B及其脂质体的效价的方法。Objective To establish a turbidimetric method for the content determination of amphotericin B and liposomal amphotericin B. Method The test organism was Saccharomyces cerevisiae and cultured with fresh medium （pH6. 5）. Adjusted spore suspension of Saccharornyces cerevisiae inoculated with a 2% -3.3% （V/V） ratio, at （37±1）℃ for 6-8 hours. Results The linear range was 0. 04-0. 10μg/ml. The precision （RSD） of the standard curve assay and two-level assay was 1.64% and 0. 38% for intra-day, 2.79% （n=8） and 0.87% （n=3） for inter-day and the average recoveries were 103.5% （n=5） and 106.6% （n=2） respectively. Conclusion The method was sensitive, rapid and feasible to determine amphotericin B and liposomal amphotericin B

关 键 词：效价 浊度法 两性霉索B 脂质体 

分 类 号：R978.5[医药卫生—药品]