哺乳动物精卵融合机制  

The Mechanism of Sperm-oocyte Fusion in Mammals

作　　者：毕聪明[1] 彭树英[2] 曹俊伟[2] 王珅[1] 

机构地区：[1]锦州医学院畜牧兽医学院,锦州121001 [2]西北农林科技大学生物工程研究所,杨凌712100

出　　处：《中国生物工程杂志》2005年第B04期41-44,共4页China Biotechnology

摘　　要：最近基因打靶研究揭示出了参与精卵结合和融合的各种分子。精子中ADAMs因子(是含 有裂解蛋白和金属蛋白酶结构域蛋白质家族),包括繁殖因子α、繁殖因子β以及cyritestin,经过 研究已经发现它们对精卵结合有重要作用,而对精卵的融合不重要。通过研究推测出其受体为 卵母细胞整合蛋白,其对精卵交互作用是必需的。最近,一些研究表明CD9和卵母细胞上GPI锚 定蛋白(glycosyl plaosphatidyl inositol糖基磷脂酰肌醇),以及精子上的附睾蛋白DE均是精卵融合 过程中的侯选因子,如果缺乏这些蛋白质分子或其作用受到干扰将导致精卵融合机制紊乱。综 述重点讨论了参与精卵交互作用的相关分子的最新研究进展。Recent studies involving gene targeting have clearly demonstrated the various molecules that are involved in sperm-oocyte binding and fusion. Sperm ADAMs （family of proteins with a disintegrin and metalloprotease domain）, including fertilinα, fertilinβ and cyritestin, have been investigated and found to be important for binding rather than for fusion and painstaking studies have raised suspicions that their putative receptors, oocyte integrins, are necessary for the sperm-oocyte interaction. Recently, several studies have focused the spotlight on CD9 and glycosylphosphatidylinositol （GPI）-anchored proteins on oocytes, and epididymal protein DE on sperm, as candidate molecules involved in sperm-oocyte fusion. Lack of, or interference with the function of, these proteins can disrupt the sperm-oocyte fusion without changing the binding. The candidate molecules involved in the sperm-oocyte interaction suggested from the recent progress in this research field was highlighted.

关 键 词：精卵融合 哺乳动物 CD9 DE ADAMS GPI 

分 类 号：Q7[生物学—分子生物学] Q959.8