失配双链核糖核酸抑制柯萨奇B3病毒感染细胞的研究  

Research on Mismatched Double Stranded RNA to Inhibit Coxsackievirus B3

作　　者：胡冬琴[1] 聂实践[1] 刘哲伟[2] 孙红妹[2] 

机构地区：[1]北京市营养源研究所,北京100054 [2]首都儿科研究所,北京100020

出　　处：《中国生物工程杂志》2005年第B04期232-234,共3页China Biotechnology

基　　金：北京市248工程项目(H010210140111)

摘　　要：通过药物抑制病毒致细胞病变效应实验和药物对病毒空斑形成抑制实验,研究低毒性失 配双链核糖核酸(Poly I:C12U)和已知的抗病毒药物双链核糖核酸聚肌胞(Poly I:C)在细胞水平对 柯萨奇病毒感染的抑制作用比较,证明Poly I:C12U的抗病毒作用。实验结果表明Poly I:C12U和 Poly I:C均可有效地抑制病毒空斑的形成,且量效关系已确定。The Hela cells were cultured with or without Mismatched Double Stranded RNA （Poly I：C12U） for 6 h at 37℃ before and after Coxsackievirus B3（CVB3） attacking. The results showed that Poly I：C12 U could protect the cells infected by CVB3, and had no protection to the cells which treated by Poly I：C12U before CVB3 attacking. The result was almost same between the Poly I：C12U and Poly I： U.

关 键 词：失配双链核糖核酸 柯萨奇B3病毒 

分 类 号：S852.659.4[农业科学—基础兽医学] S859.79[农业科学—兽医学]