鸡胚原始生殖细胞诱导分化为神经元样细胞的研究  

Study of Differentiation of Chicken Primordial Germ Cells into Neuron-like Cells

作　　者：蔡琳琳[1] 秦洁[1] 李碧春[1] 肖小珺[2] 陈国宏[1] 吴信生[1] 吴圣龙[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]湖州师范学院生命科学学院,湖州313000

出　　处：《畜牧兽医学报》2005年第12期1354-1357,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30170678)

摘　　要：Primordial germ cells(PGCs) isolated from gonads of stage 19 by Ficoll density gradient centrifugation were cultured in DMEM medium in vitro.Then the second passage PGCs were induced to differentiate into neuron-like cells by different induction media.Specific markers and structures were detected by histochemistry method.The results showed that induction medium RA and IBMX were the best ways to induce PGCs into neuron-like cells,and almost 80%-85% cells exhibited typical neuron-like phenotype after induction.Special Nissl body was found by histochemistry.The effect of induction medium RA+IBMS,RA+BHA+DMSO and RA+BHA+DMSO+IBMX were better than that of induction medium BHA+DMSO.Under the same induction medium,about 80%-85% neuron-like cells could be detected no matter what PGCs were cultured 24 h,48h or 72 h in vitro.Primordial germ cells （PGCs） isolated from gonads of stage 19 by Ficoll density gradient centrifugation were cultured in DMEM medium in vitro. Then the second passage PGCs were induced to differentiate into neuron-like cells by different induction media. Specific markers and structures were detected by histochemistry method. The results showed that induction medium RA and IBMX were the best ways to induce PGCs into neuron-like cells, and almost 80%-85% cells exhibited typical neuron-like phenotype after induction. Special Nissl body was found by histochemistry. The effect of induction medium RA＋ IBMS,RA＋BHA＋ DMSO and RA＋ BHA＋ DMSO＋ IBMX were better than that of induction medium BHA＋DMSO. Under the same induction medium, about 80 %-85 % neuron-like cells could be detected no matter what PGCs were cultured 24 h, 48h or 72 h in vitro.

关 键 词：鸡 原始生殖细胞 分化 神经元样细胞 

分 类 号：S831.1[农业科学—畜牧学]