GC_(02)球虫病疫苗的最佳免疫剂量与免疫日龄的研究  

Research of Optimum Immune Dosage and Age of GC_(02) Coccidian Vaccine

作　　者：顾有方[1] 鲍广刚[1] 陈会良[1] 蔡汉乔[1] 刘德义[1] 金光明[1] 李升和[1] 

机构地区：[1]安徽科技学院动物科学学院,凤阳233100

出　　处：《畜牧兽医学报》2005年第12期1367-1370,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：安徽省优秀青年科技基金(200106);安徽省高校中青年学科带头人培养对象基金;安徽省高等学校"十五"优秀人才项目

摘　　要：The experiments were carried out to investigate the optimum immune age and immune dosage of GC02 coccidian vaccine.Ninety healthy cross broilers were chosen and randomly divided intoⅠ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ groups（n=15） to study the immune effect of different immune dosage at the same immune times.Each chicken in Ⅰ-Ⅳ groups were orally inoculated with 4 000,8 000,16 000,20 000 sporulated oocysts of GC02 coccidian vaccine,respectively.Chickens in Ⅴ group were not infected with sporulated oocysts and chickens in Ⅵ group were not inoculated.On the 7th day after the second immunity,Ⅰ-Ⅳgroups were（orally） challenged with 1.0×105 sporulated oocysts for each chicken.On the 8th day after challenging with the sporulated oocysts,all chicken were slaughtered.According to lesion scoring and faces scoring,OPG,ACI,relative growth rate,feed conversion,etc.,the effects of different immune dosage of GC02 coccidian vaccine were evaluated.The results suggested that optimum immunization method was inoculating 8 000 sporulated oocysts per bird two times.One-hundred and eighty healthy chickens were randomly divided into A,B,C,D,E,F,G1,G2,G3 groups（n=20） to study the immune effects of different immune age with the same immune dosage.Chickens in A,B,C groups were immunized with GC02 coccidian vaccine at the different immune age;D,E,F and G1,G2,G3 were positive and negative control groups respectively,the immune and challenge is the same as experiment one.The results concluded that GC02 coccidian vaccine is safe and effectual in preventing coccidiosis,immunize with 8 000 sporalated oocysts per bird at the age of 7 days and 14 days can obtain the ideal effects.The experiments were carried out to investigate the optimum immune age and immune dosage of GC02 coccidian vaccine. Ninety healthy cross broilers were chosen and randomly divided into Ⅰ ,Ⅱ,Ⅲ,Ⅳ,Ⅴ ,Ⅵ groups（n=15） to study the immune effect of different immune dosage at the same immune times. Each chicken in Ⅰ-Ⅳ groups were orally inoculated with 4 000 ,8 000,16 000,20 000 sporulated oocysts of GC02 coccidian vaccine, respectively . Chickens in Ⅴ group were not infected with sporulated oocysts and chickens in Ⅵ group were not inoculated. On the 7th day after the second immunity, Ⅰ-Ⅳ groups were orally challenged with 1.0×10^5 sporulated oocysts for each chicken. On the 8th day after challenging with the sporulated oocysts, all chicken were slaughtered. According to lesion scoring and faces scoring, OPG, ACI, relative growth rate, feed conversion, etc. , the effects of different immune dosage of GC02 coccidian vaccine were evaluated. The results suggested that optimum immunization method was inoculating 8 000 sporulated oocysts per bird two times. One-hundred and eighty healthy chickens were randomly divided into A,B,C,D,E,F,G1 ,G2 ,G3 groups （n=20） to study the immune effects of different immune age with the same immune dosage. Chickens in A,B,C groups were immunized with GC02 coccidian vaccine at the different immune age; D,E,F and G1 ,G2,G3 were positive and negative control groups respectively, the immune and challenge is the same as experiment one. The results concluded that GC02 coccidian vaccine is safe and effectual in preventing coccidiosis, immunize with 8 000 sporalated oocysts per bird at the age of 7 days and 14 days can obtain the ideal effects.

关 键 词：鸡 球虫 疫苗 免疫剂量 免疫日龄 

分 类 号：S852.52[农业科学—基础兽医学]