经酶修饰和未经酶修饰的鹌鹑红细胞对甲壳动物血清凝集素凝集活性的差异研究  

The difference between enzyme-treated and untreated quail erythrocytes on hemagglutinating activity of quail erythrocytes with lectins

作　　者：戴聪杰[1] 陈寅山[2] 谢进金[3] 吴文林[3] 

机构地区：[1]厦门大学海洋学系近海海洋环境科学国家重点实验室,福建厦门361005 [2]福建师范大学生物工程学院,福建福州350007 [3]泉州师范学院生物系,福建泉州362000

出　　处：《海洋科学》2005年第12期35-37,55,共4页Marine Sciences

基　　金：福建省自然科学基金资助项目(B0410027);泉州市科学技术局重点科技计划资助项目(Z200239)

摘　　要：为寻找凝集素作为分子探针提供基础资料,用12种甲壳动物血清抽提液对天然的或经酶修饰的鹌鹑红细胞进行凝集试验。实验结果表明,经蜗牛酶修饰后的鹌鹑红细胞的凝集作用发生了变化,与未经酶处理的鹌鹑红细胞相比,凝集活性提高的有日本沼虾(Macro-brachium nipponense)等7种,凝集活性下降的有罗氏沼虾(Macrobrachium rosenbergi)等2种。凝集素对动物红细胞的凝集可看作是与这些细胞表面的多糖或糖蛋白———即受体的识别性结合,这表明鹌鹑红细胞表面具有12种凝集素的受体,且大多数受体都受到酶修饰的影响,从而在凝集活性上表现出差异。As to offer basic data for searching for lectins as molecular probe, the serums of twelve species of crustaceans are detected for hemagglutinating activity with untreated and enzyme-treated quail erythrocytes. The results showed that hemagglutinating activity had changed when erythrocytes were treated by enzyme. Compared with untreated quail erythrocytes, the hemagglutinating activity of enzyme-treated quail erythrocytes of seven lectins from crustaceans such as Macrobrachium nipponense was increased, and those of two lectins from crustaceans such as M. rosenbergi were declined. The hemagglutination was regarded as discriminating combination between lectins and acceptors on the erythrocyte surface. It indicated that there are different types of acceptors on the quail erythrocyte, and most of them were effected by enzyme treated and show difference in hemagglutinating activity.

关 键 词：甲壳动物 凝集素 酶修饰 凝集活性 

分 类 号：Q959.233[生物学—动物学]