斜带髭鲷养殖群体遗传多样性RAPD分析  

RAPD analysis of genetic diversity in reared Ha palogenys nitens stock

作　　者：梁君荣[1] 王军[2] 苏永全[2] 丁少雄[2] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005 [2]厦门大学海洋与环境学院,福建厦门361005

出　　处：《海洋科学》2005年第12期47-50,共4页Marine Sciences

基　　金：国家自然科学基金资助项目(30070595);福建省自然科学基金资助项目(B80010003)

摘　　要：采用随机扩增多态性DNA(RAPD)技术对广东饶平海水网箱养殖的斜带髭鲷(Hapalogenys nitens Richardson)人工苗养殖群体DNA多样性进行检测.首先从40个引物(S461～S480,S501～S520)中快速筛选出32个引物,它们均能扩增出清晰稳定的DNA片段.再将该32个引物对46尾斜带髭鲷进行RAPD分析,共检测到177个位点,其中多态位点55个(分别由18个引物获得),占31.07%.由此得出该群体的平均多态性为31.07%,平均遗传差异度为0.089,表明该养殖群体遗传多样性水平较高.Genetic diversity of DNA in reared Hapalogenys nitens stocks, which were collected from Zhelin Bay in Guangdong Province in Jun. 1999, was analyzed by random amplified polymorphic DNA（RAPD） technique in this paper. There were 177 gene loci detected from 32 primers, and fifty-five loci of them were polymorphic. So the polymorphism and the mean difference of the reared H. nitens stocks were 31.07% and 0. 089 respectively. The results showed that the reared H. nitens population still retained richer genetic diver sity.

关 键 词：斜带髭鲷(Hapalogenys nitens Richardson) 遗传多样性 随机扩增多态性DNA(RAPD) 

分 类 号：Q953[生物学—动物学] Q523