氨苯砜与牛血清白蛋白相互作用的热力学研究  

Thermodynamic Study on the Interaction between Dapsoneand Bovine Serum Albumin

作　　者：原华平[1] 关洪亮[1] 潘祖亭[1] 

机构地区：[1]武汉大学化学与分子科学学院,武汉市430072

出　　处：《光谱实验室》2005年第6期1192-1195,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金资助项目(20275028)

摘　　要：荧光光谱法和紫外-可见光谱法研究了在模拟人体生理条件下,氨苯砜和牛血清白蛋白结合反应的特征,发现氨苯砜对BSA有较强的荧光猝灭作用,且氨苯砜的紫外吸收光谱和BSA的荧光光谱有一定程度的重叠,获得其作用距离和结合过程的基本热力学参数。The binding characteristics of dapsone （DS） and bovine serum albumin （BSA） were studied by fluorescence and absorption spectroscopy in aqueous solution. DS had a powerful ability to quench the BSA fluorescence via non-radiation energy transfer mechanism. The fluorescence quenching data were analyzed according to Stern-Volmer equation and double-reciprocal equation, from which the reaction distance and the thermodynamic parameters were obtained.

关 键 词：氨苯砜 牛血清白蛋白 荧光光谱法 吸收光谱法 热力学 

分 类 号：O657.32[理学—分析化学]