诱导二倍体和同源四倍体白菜细胞质雄性不育系不定芽培养基的筛选  

The Selection of the Medium for the Adventitious Shoots Induction of Cyto- plasmic Male Sterility in Diploid and Autotetraploid Brassica campestris ssp. chinensis (L.) Makino

作　　者：孙敏红[1] 张蜀宁[1] 邓云[1] 

机构地区：[1]南京农业大学蔬菜研究所,南京210095

出　　处：《植物生理学通讯》2005年第6期741-744,共4页Plant Physiology Communications

基　　金：江苏省"十五"科技攻关项目(BE2001321-1)

摘　　要：以二倍体和四倍体白菜细胞质雄性不育系的子叶为外植体,采用正交设计研究TDZ(0.1、0.5、1.0mg·L-1)、NAA(0.1、0.5、0.8mg·L-1)和AgNO3(0、5、10mg·L-1)三因素三水平诱导不定芽的结果表明:二倍体的不定芽再生优化培养基为MS+1.0mg·L-1TDZ+0.8mg·L-1NAA+5mg·L-1AgNO3(其再生率为80.7%),三因素影响的顺序为:NAA>AgNO3>TDZ;四倍体的不定芽再生优化培养基为MS+0.5mg·L-1TDZ+0.5mg·L-1NAA+10mg·L-1AgNO3(再生率为83.3%),三因素影响的顺序为:AgNO3>NAA>TDZ。后者所需TDZ/IAA的比值低于前者。Using cotyledons of cytoplasmic male sterility （CMS） in diploid and autotetraploid Brassica campestris ssp. chinensis （L.） Makino as explant, the effects of TDZ （0.1, 0.5 and 1.0 mg·L^-1）, NAA （0.1, 0.5 and 0.8 mg·L^-1） and AgNO3 （0, 5.0 and 10.0 mg·L^-1） on adventitious shoot induction were studied by L9（3^4） orthogonal design, The experimental results showed that the optimal medium for adventitious shoot induction of 2x CMS was MS ＋ 1.0 mg·L^-1 TDZ＋0.8 mg·L^-1 NAA＋5.0 mg·L^-1 AgNO3, and the frequency of adventitious shoot regeneration was 80.7%, the sequence of effecting factors was NAA〉AgNO3〉TDZ. The optimal medium of 4x CMS was MS＋0.5 mg·L^-1 TDZ＋0.5 mg·L^-1 NAA＋10.0 mg·L^-1 AgNO3, and the frequency of adventitious shoot regeneration was 83.3%, the sequence of effecting factors was AgNO3〉NAA〉TDZ. The ratio on TDZfIAA of autotetraploid was lower than that of diploid.

关 键 词：白菜 同源四倍体 细胞质雄性不育系(CMS) 正交设计 不定芽诱导 

分 类 号：S634.3[农业科学—蔬菜学]