中药植物黄山药发根基因的遗传转化  

Genetic Transformation of Dioscorea panthaica with Agrobacterium rhizogenes

作　　者：顾月华[1] 李艳[1] 

机构地区：[1]中国科学技术大学生命科学学院,合肥230027

出　　处：《植物生理学通讯》2005年第6期755-757,共3页Plant Physiology Communications

摘　　要：以发根农杆菌菌株A4转染已预培养1d的黄山药茎段后,共感染3d,其转化效果最佳;转化毛状根在无生长调节物质的MS培养基上培养可获得丛状芽,并发育成植株。The perfect efficiency of genetic transformation of Dioscorea panthaica stem from pre-cultivation 1 d and co-cultivation 3 d, was got by the combination of strain A4 and Agrobacterium rhizogenes. Clustered shoots could grow from hairy roots in MS medium without growth regulators and then could form regenerated plants.

关 键 词：黄山药 发根农杆菌 转化 再生植株 

分 类 号：S567.9[农业科学—中草药栽培]