中国樱桃品种“对樱”再生体系的优化和转基因初探  

Preliminary Study on the Optimization of Regeneration and Genetic Transfor- mation of Chinese Cherry (Prunus pseudocerasus Lindl. cv. Duiying)

作　　者：赵玉辉[1] 郭印山[1] 周宇[2] 张开春[2] 李作轩[1] 王力华[3] 

机构地区：[1]沈阳农业大学园艺学院,沈阳110061 [2]北京市农林科学院林业果树研究所,北京100093 [3]中国科学院沈阳应用生态研究所,沈阳110016

出　　处：《植物生理学通讯》2005年第6期770-772,共3页Plant Physiology Communications

基　　金：北京市自然科学基金重点项目(6041002)

摘　　要：优化了中国樱桃品种“对樱”不定根离体再生体系,用农杆菌介导法,将抗菌肽B基因导入“对樱”,通过抗性筛选获得了44株抗性转化植株。PCR检测有9株为阳性,初步说明抗菌肽基因已整合到“对樱”基因组中。The system of regeneration in vitro from adventitious roots in Chinese cherry （Prunus pseudocerasus Lindl. cv. Duiying） was optimized. The antibacterial peptide B gene was transformed to cherry with Agrobacterium tumefaciens-mediated transformation system. The 44 plants regenerated which had resistance to Gent were deteced by PCR, and 9 plants were positive. It indicated into the genome of cherry. that the antibacterial peptide gene had been integrated

关 键 词：中国樱桃 抗菌肽 转基因 

分 类 号：S662.5[农业科学—果树学]