不同时间电针干预对脑缺血再灌注大鼠学习记忆能力及mAchR的影响  

Influence of Different-time Electracupuncture Intervention on Learning and Memory Adilities and mAchR in Rats with Cerebral Ischemia and Reperfusion

作　　者：方程[1] 

机构地区：[1]上海中医药大学,上海201203

出　　处：《上海针灸杂志》2005年第12期36-39,共4页Shanghai Journal of Acupuncture and Moxibustion

摘　　要：目的研究电针对脑缺血再灌注大鼠学习记忆功能的影响,比较缺血前电针干预和缺血后超早期电针治疗对大鼠的影响。方法用颈内动脉线栓法制脑缺血再灌注模型。术前、术后电针组分别在手术前、缺血后超早期予30 m in的电针治疗。采用避暗法,受体的放射配体结合分析法观察电针对大鼠避暗试验和海马胆碱能M-受体(mAchR)结合量的影响。结果脑缺血1.5 h再灌注12 h后,模型组大鼠避暗试验潜伏期缩短(215.00±69.498 s),错误次数增加(0.90±0.316),海马mAchR结合量降低(362.24±162.53 fmol/mg)(P<0.05)。电针干预可以延长避暗试验潜伏期,减少错误次数(P<0.05),术前电针可增加海马mAchR结合量(518.62±165.35 fmol/mg)(P<0.05)。结论电针特别是术前电针预处理可以改善缺血模型大鼠的学习记忆功能。术前电针预处理可以明显升高缺血再灌注损伤后所致的异常降低的mAchR结合量,从而改善中枢胆碱能系统功能,在缺血性脑血管病防治中有潜在应用价值。尚不能认为缺血前电针预处理与缺血后电针治疗脑缺血的作用有差别。Objective To investigate the influence of electracupuncture on learning and memory adilities in rats with cerebral ischemia and reperfusion and compare the effects on the rats between pre-iscbemic electracupuncture pretreatment and post-iscbemic super-early electracupuncture. Method A model of cerebral ischemia and reperfusion was made by intracarotid thread occlusion. The preoperative and postoperative groups were treated with electroacupuncture for 30 min before the operation and in the super-early stage after ischemia, respectively. The influences on the darkness-avoiding test and hippocampi mAchR binding quantity were observed by a darkness-avoiding method and receptor-radioactive ligand binding analysis, respectively. Results Mter 1.5 hrs cerebral iscbemia and 24 hrs reperfusion, the darkness-avoiding test showed a shorted latent period （215.00 ±69.498 s） and an increase in the number of errors （0.90 ±0.316）, and hippocampi mAchR binding quantity decreased （362.24 ±162.53 fmol/mg） （P 〈0.05）, in the model group of rats. Electroacupuncture intervention could prolong the latent period and decrease the number of errors. Preoperative electroacupuncture could increase hippocarnpi mAchR binding quantity （518.62 ± 165.35 fmol/mg） （P 〈 0.05 ）. Conclusion Electroacupuncture, especially its, can improve learning and memory adilities in a rat model of iscbemia. Preoperative electroacupuncture pretreatment can significantly increase mAchR binding quantity lowered abnormally after injury due to ischemia and reperfusion to improve the function of the central cholinergic system, which is of the potential value of application in preventing and treating ischemic cerebrovascular diseases. It can not be considered that there was a difference in the effect on cerebral ischemia between pre-ischemic electroacupuncture pretreatment and post-iscbemic electroacupuncture.

关 键 词：脑缺血再灌注 学习记忆功能 电针 预处理 

分 类 号：R2-03[医药卫生—中医学]