三磷酸腺苷-氯化镁对兔肺再灌注损伤的保护作用  

Protective Effects of ATP-MgCl_2 on Reperfusion Injury in Rabbit Lung Perfusion Model

作　　者：安君[1] 谭滨[2] 谷春久[1] 马支媛[2] 张宁[2] 金基焕[3] 

机构地区：[1]中国医科大学附属第一院心脏外科,辽宁沈阳110001 [2]中国医科大学附属第一院麻醉科,辽宁沈阳110001 [3]延边大学医学院附属医院心胸外科,吉林延边133001

出　　处：《中国体外循环杂志》2005年第4期223-225,共3页Chinese Journal of Extracorporeal Circulation

摘　　要：目的利用家兔左肺灌注模型,探讨三磷酸腺苷-氯化镁(ATP-MgC l2)对缺氧再灌注肺线粒体内钙离子浓度和肺组织水分的影响。方法观察ATP-MgC l2对再灌注肺组织线粒体内钙离子浓度和肺组织水分的影响。结果(1)家兔左肺灌注模型均获成功。(2)缺氧再灌注组线粒体内钙离子浓度显著高于正常对照和缺氧组(P<0.01);ATP-MgC l2加用再灌注组线粒体内钙离子浓度显著低于缺氧再灌注组(P<0.01)。(3)缺氧再灌注组肺组织湿干重比值(LW/D R)显著高于正常对照和缺氧组(P<0.01);ATP-MgC l2加用再灌注组LW/D R显著低于缺氧再灌注组(P<0.01)。结论本模型具有操作简便、成功率高的优点,是可行的肺离体研究模型;ATP-MgC l2明显抑制家兔缺氧再灌注肺线粒体内钙沉积和肺组织水肿,提示ATP-MgC l2对肺缺血再灌注损伤具有保护作用。OBJECTIVE The purpose of this study is to establish reliable and appropriate rabbit left lung perfusion model and to clarify whether ATP - MgCl2 can influence calcium concentration in mitochondria during lung hypoxia/reperfusion injury in the model. METHODS First, we established rabbit single left lung perfusion model. Second, we use this model to clarify influence of ATP - MgCl2 on calcium concentration in mitochondria during lung reperfusion injury. RESULTS （ 1 ） Rabbit left lung perfusion model were successfully established in all 30 cases. （2） The free calcium concentration in mitochondria in hypoxia/reperfusion group was significantly higher than that in normal control and hypox- ia groups （ P 〈 0.01 ） ; In ATP - MgCl2 added reperfusion group was significantly lower than that in hypoxia/reperfusion group （ P 〈0.01 ） ; Lung wet/dry weight ratio in ATP - MgCl2 added reperfusion group was less than that in hypoxia and hypoxia/reperfusion groups （ P 〈 0.01 ）. CONCLUSION The rabbit left lung perfusion model is a useful and reliable model for lung research. ATP - MgCl2 may have protective effect on the lung hypoxia/reperfusian injury through inhibition of mitochondrial calcium accumulation and reduction of lung tissue edema.

关 键 词：兔 肺 线粒体 再灌注损伤 ATP-MGCL2 

分 类 号：R654.1[医药卫生—外科学]