大豆疫霉(Phytophthora sojae)单卵孢株毒性纯系的建立  

Development of virulent pure line of Phytophthora sojae through single-oospore progeny segregation

作　　者：杨明秀[1] 文景芝[1] 李永刚[1] 刘春来[1] 李莹[1] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《东北农业大学学报》2005年第6期702-706,共5页Journal of Northeast Agricultural University

基　　金：黑龙江省博士后基金资助(LRB00064)

摘　　要：将培养30d的大豆疫霉1号生理小种卵孢子在适宜条件下诱导萌发,随机挑取30个单卵孢株进行纯培养和毒力测定,在测定结果与亲代一致(1号生理小种)的菌株中随机选取1个单卵孢株重复上述试验。试验经过连续两代单卵孢株分离培养,获得了毒力稳定表达的大豆疫霉1号生理小种单卵孢株毒力纯系,为开展大豆疫霉毒性遗传和变异的分子生物学研究提供了研究材料。30 single-oospore isolates obtained from race 1 of Phytophthora sojae （Ps-H2 strain） were evaluated using hypocotyls-inoculation method for race phenotype on soybean cultivars. One race 1 line with desired phenotypes was selected randomly for further experimentation. The pure-line in virulence was obtained after the second generation. This study demonstrated that the pure-breeding race of P.sojae could be developed by repeatedly self-fertilizing of oospore progeny. From this study, we got molecular research material of Phytophthora sojae on virulent inheritance and variation.

关 键 词：大豆疫霉 纯系 毒力 单卵孢株 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治] S565.1[农业科学—植物保护]