以胶原为载体的蛋白药物体内释放和药代动力学的研究  

Protein Release from Implantable Collagen Carrier and Pharcodynamics Study

作　　者：丁时踽[1] 刘玲蓉[1] 李学敏[1] 张其清[1] 

机构地区：[1]中国医学科学院中国协和医科大学生物医学工程研究所,天津300192

出　　处：《中国生物医学工程学报》2005年第6期775-779,共5页Chinese Journal of Biomedical Engineering

基　　金：国家"863"计划项目(2002AA326040)

摘　　要：复合细胞因子引导组织再生修复材料作为一种特殊的蛋白药物一载体释放体系,为了解其在体内降解释放的药物动力学基本性质,以进一步完善材料制备工艺并更好地控制蛋白药物的局部浓度,本研究通过125I-BSA/胶原基复合材料的体内植入释放来模拟这种释放体系的体内动态过程,得到了125I-BSA的释放曲线(AUC=323%×d,MRT=4.83d)。本研究还对药物/材料的复合工艺作了改进,体内释放试验结果显示,用壳聚糖包裹125I-BSA生成胶体颗粒,再和胶原材料复合,可明显增强其缓释作用,更有效地延长药物在植入局部存留的时间(AUC=476%×d,MRT=7.22d)。上述两种复合材料在体外药物释放试验的数据分别与体内试验的结果有一定的相关性。Tissue regeneration inductive materials compounded with cytokine have been the focus in the study of biomaterial and tissue-engineering for decades. The objective of this study was to evaluate the in vivo release character of a protein-carrier delivery system using ^125I-BSA/collagen sponge complex as a model（AUC = 323 % × day, MRT = 4.83days）and thus give basic information of its in vivo pharmacokinetics for the purpose of improving the preparation of the protein-inductive materials compunding technique. One kind of improvement on the technique was achieved. It was found that the complex compounded with ^125I-BSA encapsulated by chitosan microparticles exhibited a flatter release curve compared with that not encapsulated by chitosan（ AUC = 476% × day, MRT = 7.22days）. The controlled release effect to prolong the local retention of the protein after implantation was enhanced. In vitro release experiments gave results in consistence with those in vivo.

关 键 词：胶原 ^125I-BSA 体内药物动力学 壳聚糖 

分 类 号：R318.08[医药卫生—生物医学工程]