氯化锂对染铅大鼠体重及学习记忆的影响  

Effects of lithium on lead-exposed rat body weight and learning ability and memory capacity

作　　者：杨芳[1] 李积胜[1] 王延坤[1] 王大宁[1] 

机构地区：[1]武警医学院军事预防医学研究所,天津300162

出　　处：《毒理学杂志》2005年第4期269-271,共3页Journal of Toxicology

基　　金：国家自然科学基金资助(39870689)

摘　　要：目的探讨氯化锂对染铅大鼠体重和学习记忆能力的影响,进一步阐明锂对铅神经毒性的拮抗效应及其可能机制。方法将大鼠随机分为对照组、染铅组和4个铅+氯化锂(3、30、300和3 000 mg/kg)组,分别给予普通饲料和含氯化锂(3、30、300和3 000 mg/kg)的饲料喂养60 d,通过测量体重和Y-迷宫实验,采用NADPH-d组织化学染色,观察各组大鼠之间体重增量,学习记忆能力和大脑皮层一氧化氮合酶(NOS)阳性细胞数的差别。结果染铅组大鼠体重增量低于对照组,Y-迷宫实验学会次数高于对照组(P<0.01);铅+氯化锂(3和30 mg/kg)组大鼠体重较染铅组大鼠增长多(P<0.01),铅+氯化锂(3、30和300 mg/kg)组Y-迷宫学会次数均低于染铅组大鼠(P<0.01)。染铅组皮层NOS阳性细胞数较正常大鼠明显减少(P<0.05);与染铅组比较,铅+氯化锂(3、30、300和3 000 mg/kg)组皮层NOS阳性细胞数均显著性增多(P<0.05)。结论锂能明显促进染铅大鼠体重的增加和学习记忆能力的增强,锂对铅的神经毒性有一定拮抗作用。Objective To investigate the antagonistic of lithium on the neurotoxicity of lead acetate and the possible mechanism,we observed the effects of lithium chloride on the body weight, learning ability and memory capacity of lead-exposed rats. Methods Wistar rats were randomly divided into control group, lead gorup, and four lead ＋ lithium chloride （LiCl） （3,30,300 and 3 000 mg/kg）groups. Four lead ＋ LiCl groups were feed with food containing 3,30,300 and 3 000 mg/kg LiCl respectively. By means of measuring the body weight, using Y-maze test and NADPH-d histochemistry,we observed the difference of the learning ability, memory capacity of all groups and the brain cortex activation of cortex NOS. Results Results of body weight gain and Y-maze：Lead gorup was weaker than Cont group（ P 〈 0.05）;lead ＋ LiCl（3,30 and 3 000 mg/kg）groups were better than lead gorup （P 〈 0.05）;Results of NADPH-d histochemistry：the number of NOS positive neurons of lead groups were lower than Cont group （ P 〈 0.05） ; the number of NOS positive neurons of lead ＋ LiCl（3,30 and 3 000 mg/kg）groups were higher than lead group（ P 〈 0.05）. Conclusion Lithium could increase the body weight and improve the ability of learning and memory of lead-exposed rats. Lithium could resist the lesion of nervous system induced by lead obviously.

关 键 词：锂 铅 体重 学习记忆 

分 类 号：R99[医药卫生—毒理学]