含报告基因GFP的hGH真核表达载体的构建  

Construction of eukaryotic expression vector of human growth hormone

作　　者：宋晓妍[1] 袁云[1] 闫志勇[1] 张娓[1] 张钦宪[1] 

机构地区：[1]郑州大学医学院组织学与胚胎学教研室,河南郑州450052

出　　处：《河南医学研究》2005年第4期304-305,共2页Henan Medical Research

摘　　要：目的:构建含报告基因GFP的人生长激素(hGH)的真核表达质粒。方法:利用分子生物学技术,将hGH目的片断连接于含绿色荧光蛋白的真核表达质粒pcDNA3.1(pcGFP)中并测定序列。结果:核酸序列测定证实本实验所构建的质粒是成功的。结论:构建的重组真核细胞表达质粒pcGH-GFP为进一步研究GH在体内的生理作用和病理作用有一定意义。Objective： To construct the eukaryotic expression vector pcGH-GFP. Methods： Using gene Recombinant technique, the DNA fragment of hGH gene was constructed into the eukaryotic expression vector pcGFP. The inserted target gene in the eukaryotic expression vector was verified by nucleotide sequencing. Results ： The eukaryotic expression vector was constructed successfully. Conclusion ： The recombinant plasmid including human growth hormone possesses a certain significance for the study of its physical and pathological role in vivo.

关 键 词：人生长激素 载体 基因重组 

分 类 号：Q575.11[生物学—生物化学]