CEA真核表达载体的瞬时表达及检测  

Transient expression of eukaryotic expression plasmid CEA in vitro and its detection

作　　者：袁云[1] 宋晓妍[1] 张娓[1] 闫志勇[1] 张钦宪[1] 

机构地区：[1]郑州大学基础医学院组织学与胚胎学教研室,河南郑州450052

出　　处：《河南医学研究》2005年第4期306-308,共3页Henan Medical Research

摘　　要：目的:检测含癌胚抗原(CEA)的真核表达质粒pWACEA在体外的瞬时表达。方法:用脂质体包裹含CEA的真核表达质粒pWACEA转染MCF7细胞,采用原位杂交和免疫组化技术检测CEA的表达。结果:转染细胞原位杂交和免疫组化均为阳性;对照标本未见阳性信号。结论:真核表达质粒pWACEA在体外可表达CEA分子,为进一步建立CEA阳性动物肿瘤细胞模型及研究CEA阳性肿瘤的免疫防治提供了基础。Objective： To detect expression of the eukaryotic expression plasmid pWA-CEA in vitro. Methods： MCF-7 cell line was transfected by this mammalian expression plasmid pWA-CEA using lipofectamine reagent. The expression of CEA molecule was detected by in situ hybridization and immunocytochemistry technique. Results ： The positive signals were found in MCF-7 cells, but no positive signals were exhibited in contrast specimens. Conclusion ： CEA molecule can be expressed in vitro after transfection, which lay a good foundation for constructing animal CEA positive tumor cell model and studying the prevention and therapy of CEA positive tumor.

关 键 词：癌胚抗原 质粒表达 原位杂交 

分 类 号：Q786[生物学—分子生物学]