The Evolutionary Relationship of the Domain Architectures in the RhoGEF-containing Proteins  

作　　者：Qing-Lan Sun Hong-Jun Zhou Kui Lin 

机构地区：[1]The Key Laboratory of the Ministry of Education for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing 100875, China.

出　　处：《Genomics, Proteomics & Bioinformatics》2005年第2期94-106,共13页基因组蛋白质组与生物信息学报（英文版）

基　　金：We thank anonymous reviewers for their helpful comments.This work was supported by the National High-Tech Research and Development Program of China(Grant No.2003AA231030);the Excellent Young Teachers Program of the Ministry of Education of China(2003),and Beijing Normal University.

摘　　要：Domain insertions and deletions lead to variations in the domain architectures of the proteins from their common ancestor. In this work, we investigated four groups of the RhoGEF-containing proteins from different organisms with domain architectures RhoGEF-PH-SH3, SH3-RhoGEF-PH, RhoGEF-PH, and SH3-RhoGEF defined in the Pfam database. The phylogenetic trees were constructed using each individual domain and/or the combinations of all the domains. The phylogenetic analysis suggests that RhoGEF-PH-SH3 and SH3-RhoGEF-PH might have evolved from RhoGEF-PH through the insertion of SH3 independently, while SH3- RhoGEF of proteins in fruit fly might have evolved from SH3-RhoGEF-PH by the degeneration of PH domain.Domain insertions and deletions lead to variations in the domain architectures of the proteins from their common ancestor. In this work, we investigated four groups of the RhoGEF-containing proteins from different organisms with domain architectures RhoGEF-PH-SH3, SH3-RhoGEF-PH, RhoGEF-PH, and SH3-RhoGEF defined in the Pfam database. The phylogenetic trees were constructed using each individual domain and/or the combinations of all the domains. The phylogenetic analysis suggests that RhoGEF-PH-SH3 and SH3-RhoGEF-PH might have evolved from RhoGEF-PH through the insertion of SH3 independently, while SH3- RhoGEF of proteins in fruit fly might have evolved from SH3-RhoGEF-PH by the degeneration of PH domain.

关 键 词：protein domain domain architecture phylogenetic analysis domain loss RhoGEF-containing proteins 

分 类 号：Q51[生物学—生物化学]