Cloning and sequence analysis of atom gene from Sclerotimia sclerotiorum  

作　　者：YANG Qian YU Han-ying LI Chang-yin 

机构地区：[1]Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, P. R. China [2]Department of Petroleum Engineering, Daqing Petroleum Institute, Daqing 163318, P. R. China

出　　处：《Journal of Forestry Research》2005年第4期260-264,共5页林业研究（英文版）

摘　　要：An atom gene was cloned from genomic DNA of Scleortinia sclerotiorum by inverse PCR. The evolutionary relationships of S. sclerotiorum and other fungi in atom gene were studied. Results showed that the atom gene from of S. sclerotiorum has a single open reading frame of 4 773 bp and does not include any introns. The derived amino acid sequence consists of 1 590 residues, and it is homologous to all fungal AROM proteins studied so far. The theoretical isoelectric point （pl） and molecular weight （Mw） is 6.5 and 172.66 kD, respectively GC percentage of the arom gene is 44.94. According to the results of searching from CDD and Prosite database, AROM protein of S. sclerotiorum contains five conserve domains： 3-dehydroquinate synthase domain, 3-dehydroquinate dehydratase （3-dehydroquinase） domain, shikimate 5-dehydrogenase domain, shikimate kinase domain, and -enolpyruvylshikimate-3-phosphate synthase （EPSP sythase） domain, and four motifs： two EPSP synthase signatures, dehydroquinase class I active site, shikimate kinase signature. According to the PIR Site Rule PIRSR000514-1, four functionally important amino acid residues are found by alignment. Putative TATA box and CAAT box locate separately in -23 and -77 loci in 5＇ un-translated region, and two loci found in downstream atom gene are likely polyadenylation signals. In addition, phylogeny of atom gene is analyzed.arom 基因被反的 PCR 从 Scleortinia sclerotiorum 的 genomic 脱氧核糖核酸克隆。S 的进化关系。在 arom 基因的 sclerotiorum 和其它真菌我们再学习。结果出现了从 5 的 arom 基因。sclerotiorum 让一个单身者打开 4 773 bp 的读的框架并且不包括任何 introns。导出的氨基酸顺序由 1 590residues 组成，并且它对蛋白质到目前为止学习了的所有真菌的 AROM 相应。理论等电点(pI ) 和分子量(Mw ) 分别地是 6.5 和 172.66 kD。arom 基因的 GC 百分比是 44.94。根据从 CDD 和 Prosite 数据库寻找的结果， S 的 AROMprotein。sclerotiorum 包含五保存领域：3-dehydroquinate synthase 领域， 3-dehydroquinate dehydratase (3-dehydroquinase ) 领域， shikimate 5 脱氢酶领域， shikimatekinase 领域，和 -enolpyruvylshikimate-3-phosphate synthase (EPSP sythase ) 领域，和四个主题：二 EPSP synthase 签名， dehydroquinase 一级活跃地点， shikimate kinasesignature。根据 PIR 地点规则 PIRSR000514-1，四机能上地重要的氨基酸残余被排列发现。通常认为的 TATA 盒子和 CAAT 盒子在 5' 在 -23 和 -77loci 独立定位在下游的 arom 基因发现的未翻译的区域，和二 loci 是 likelypolyadenylation 信号。另外， arom 基因的发展史被分析。

关 键 词：Sclerotinia sclerotiorum ATOM Gene cloning 

分 类 号：S188[农业科学—农业基础科学]