CpG-ODN对慢性乙型肝炎患者外周血树突状细胞表型和功能的影响  

Effect of CpG-ODN on the phenotype and function of monocyte-derived dendritic cells in chronic hepatitis B.

作　　者：向晓星[1] 周霞秋[1] 王俊学[2] 谢青[1] 蔡雄[2] 俞红[1] 周惠娟[1] 

机构地区：[1]上海瑞金医院感染科,上海200025 [2]上海长征医院感染科,上海200003

出　　处：《临床肝胆病杂志》2005年第6期336-338,共3页Journal of Clinical Hepatology

基　　金：上海市科委科研计划项目(编号:044119624)

摘　　要：研究含非甲基化CpG基序的寡核苷酸(CpG-ODN)对慢性乙型肝炎患者(CHB)外周血树突状细胞(den- dritic cell,DC)表型和功能的影响。以CpG-ODN刺激CHB和健康者外周血单核细胞衍生的DC成熟,结果显示,与 non-ODN和PBS组比,CpG-ODN能明显提高CHB患者DC的HLA-DR、CD86表达,使其IL-12分泌增加,刺激T 细胞增殖的能力亦显著增强(P<0．05),但不能明显提高CD1a表达。因此,CpG-ODN与TNF-α一样能促进CHB 患者外周血DC成熟进瓶增强其抗原提呈功能;CHB患者的細胞因子环境可能是DC功能沉默的重要原因。To investigate the effect of immunostimulatory CpG - ODN on the phenotype and function of dendritic cells （DC） in patients with chronic hepatitis B （CHB）. CpG- ODN and other control reagents such as TNF-α and non - ODN were added respectively to the culture medium of monocyte - derived DC in CHB and heahhy volunteers. Compared with control groups of non - ODN and PBS, the expression rates of HLA - DR and CD86 of DC in CHB with the treatment of CpG- ODN were obviously in- creased, the IL- 12 level was ＇also raised, and the stimulating capacity of DC to allogenic T lymphocytes in mixed lymphocyte reaction（MLR） was improved significantly （ P 〈 0. 05） ; In conclusion, CpG - ODN, like TNF - a, has remarkable stimulatory effect on the phenotype and function of monocyte- derived DC in patients with CHB. The disorder of expression levels of plasma TGF - β and IFN -γ in CHB might be responsible for the functional silence of DC.

关 键 词：慢性乙型肝炎 树突状细胞 非甲基化CpG基序 成熟 

分 类 号：R512.62[医药卫生—内科学]