干扰素α对慢乙肝患者肝纤维化及肝细胞凋亡的临床观察  

The clinic study of interfern alpha on liver flbrosis of patients with chronic hepatitis B

作　　者：于宏[1] 曹志宸[1] 耿建英[1] 孙小云[1] 贺占国[1] 王政民[1] 王中华[1] 

机构地区：[1]白求恩国际和平医院肝病科,河北石家庄050082

出　　处：《临床肝胆病杂志》2005年第6期363-365,共3页Journal of Clinical Hepatology

摘　　要：观察干扰素α对慢乙肝患者肝组织纤维化程度的影响。16例肝组织病理诊断符合S3-S4期患者,干扰素治疗期前后三次行肝组织炎症程度及纤维化程度病理分析,免疫组化检查肝组织TGF-β1蛋白、Fas及HBcAg抗原,TUNEL方法检查肝细胞凋亡。干扰素连续6个月治疗肝组织炎症及纤维化程度逐渐改善;在S1-S4期,Fas、 TGF-β1显著表达;干扰素α治疗3个月后Fas抗原、TGF-β1表达程度显著下降,P<0．05;细胞凋亡程度减轻P< 0．05;肝组织HBcAg表达程度无显著改变。干扰素α有显著改善S3-S4期患者肝组织肝细胞凋亡和肝纤维化程度,但需要持续的疗程。To study the clinic effection of interferon - alpha on liver fibrosis of chronic viral hepatitis B. 16 case at pathological Change S3 - S4 degrees were treated by interfem alpha for 6 - 9 months. During therapy the degree of liver fibrosis and inflammation were tested. The expression of Fas, transforming growth faetorβ1 （TGF/β1 ） and HBcAg in liver tissues were detected by immunohistochemistry. DNA fragmentation was detected by in situ terminal deoxynueleotidyl transferase mediated dUTP nick end labeling （TUNEL）. The degrees of liver fibrosis and apaptosis of hepatocytes were alleviated gradually after therapy of Interferon alpha for 3 - 9 months. The damage of DNA in hepatocytes of CHB correlated closely with the expression of Fas and TGF2 β1 in liver tissue. In severe type CHB and the early period of liver cirrhosis, the degree of liver fibrosis and the expression of Fas and TGFβ1 were high, after therapy the degrees were less. Interferon a may prevent and inhibit liver fibrosis with persistent treatment of interferona alpha.

关 键 词：乙型肝炎 干扰素Α 肝纤维化 细胞凋亡 

分 类 号：R512.62[医药卫生—内科学]