花生种皮抗黄曲霉差异基因表达初步分析(英文)  

Primary Expression Analysis of Differential Genes in Peanut Seed Capsule with Resistance to A.flavus

作　　者：单世华[1] 严海燕[2] 李春娟[1] 万书波[3] 

机构地区：[1]山东省花生研究所,山东青岛266100 [2]中科院武汉植物园研究中心,湖北武昌4300742 [3]山东省农业科学院,山东济南250100

出　　处：《花生学报》2005年第4期21-24,共4页Journal of Peanut Science

基　　金：山东省农业科学院博士后基金项目;农业部"948"项目(2003-T12)

摘　　要：黄曲霉不同抗性花生种皮存在显著差异,本试验选择经鉴定的高抗黄曲霉花生种质J11与高感种质金花1012为研究材料,以金花1012为对照利用基因芯片技术开展了抗病差异基因表达分析研究。试验共使用61078杂交探针组,结果表明,共有417个差异基因表达量上升,650个差异基因表达量下降。另外,获得抗Phytophathora Sojae真菌病害表达量上升差异基因4个,表达量下降差异基因49个。获得抗Heterodera Glycines Ichinohe真菌病害表达量上升差异基因4个,表达量下降差异基因25个。There were significant different in genes expression of peanut germ plasmas seed capsule with different resistance to A. flavus. High resistance, J-11, and high sensitive, Jinhua 1012. to A. flavus evaluated were selected, and genechip technology was used to carry out differential anti-fungus genes expression analysis. In this experiment, 61078 probe set was used and Jinhua 1012 were taken as CK. Re sults shbwed that there were 417 differential genes expressed increased, and 650 genes expressed decreased in seed capsule of J-11. In addition, compare with Jinhua 1012, there were 4 genes expressed increased, 49 genes expressed decreased resistance to Phytophthora Sojae in seed capsule of J-11. And, there were 4 genes expressed increased, 25 genes expressed decreased resistance to Heterodera glycines in seed capsule of J-11.

关 键 词：花生 差异基因表达分析 基因芯片技术 

分 类 号：S565.2[农业科学—作物学] Q78[生物学—分子生物学]