花生胚小叶外植体再生影响因素研究简报  

Primary Research of Influence Factors on Cotyledon Leaflet Regeneration of Peanut

作　　者：李春娟[1] 单世华[1] 万书波[1] 许婷婷[1] 严江[1] 姜东亮[2] 姜常松 

机构地区：[1]山东省花生研究所,山东青岛266100 [2]海阳市农技站,山东海阳265100

出　　处：《花生学报》2005年第4期36-38,共3页Journal of Peanut Science

基　　金：青岛市科技局重点实验室项目(04-2-JS-123);山东省农科院博士后基金项目及农业部"948"项目(2003-T12)

摘　　要：胚小叶外植体再生是进行花生外源基因遗传转化的重要途径之一。研究表明,品种对花生胚小叶外植体再生有较大影响,BA/NAA比值与愈伤组织诱导、芽再生数量及速度关系密切,发芽势强的花生品种宜用低BA/NAA比值的MS培养基,发芽势弱的品种则宜用高BA/NAA比值MS培养基。Explants regeneration of peanut cotyledon leaflets was one of the main method to carry out genes transformation and had many successful examples in former research. Research results showed that peanut varieties had obvious effect on leaflet regeneration. And BA/NAA ratio had important relationship with callus induction and induced buds number. Low BA/NAA ratio MS medium should be selected in cotyledon leaflets regeneration of high vigor peanut varieties, and high BA/NAA ratio MS medium should be selected in low vigor peanut varieties.

关 键 词：花生 胚小叶 再生 影响因素 

分 类 号：S565.203[农业科学—作物学]