利用模拟代谢活化体系研究污染物的基因毒性  

Gene Toxicity of Pollutants by Utilizing the Chemical Model of Metabolic Activation System

作　　者：吴新国[1] 严炜 徐靖[1] 

机构地区：[1]武汉大学资源与环境科学学院,湖北武汉430079

出　　处：《武汉大学学报（理学版）》2005年第6期719-722,共4页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金资助项目(20377032)

摘　　要：利用二氯乙烷、三氯乙烷、三氯乙烯、N,N-二甲基甲酰胺、丙酮作为典型污染物与Fe2+/EDTA/H2O2/联氨模拟体系反应,所产生的活性中间体与4-(对硝基苄基)吡啶反应生成了紫红色物质.据此,本文建立了一种分光光度法检测活性中间产物的新方法.该方法测量灵敏度高(10-6mol/L).应用该方法证实了其中4种污染物(二氯乙烷、三氯乙烷、三氯乙烯、N,N-二甲基甲酰胺)具有形成DNA加合物的能力.同时对代谢活化体系的各种显色条件进行了优化.As typical pollutants, dichloroethane, trichloromethane, trichloroethylene,N,N-Dimethylformamide and acetone were selected to react with the mimic system of Fe2＋/EDTA/H2O2/hydrazine, and the reactive intermediates formed. Further reaction of the reactive intermediates and 4-（4-nitrobenzyl）pyridine gave a red-purple colored substance. Based on this, a new method for the detecting of the reactive intermediates was established with a high sensitivity of 10^-6 mol/L. Through this method, it is verified that dichloroethane, trichloromethane, trichloroethylene, N, N-dimethylformamide have the ability to form DNA adducts. A variety of colouring conditions for the metabolic activation system were also optimized.

关 键 词：代谢活化 化学模拟 4-硝基苄基吡啶 

分 类 号：Q55[生物学—生物化学]