人生长素基因的合成及克隆  

Gene Synthesis and Cloning of Human Growth Hormone

作　　者：卢希彬[1] 卢步峰[2] 郭礼和[2] 戴平[1] 

机构地区：[1]华东师范大学,上海200062 [2]中国科学院上海生命科学研究院生物化学与细胞生物学研究所

出　　处：《生物技术通报》2005年第6期69-72,共4页Biotechnology Bulletin

摘　　要：根据人生长素基因的氨基酸序列,选择大肠杆菌所偏好的密码子,人工设计并合成了20个寡核苷酸片断。通过重叠区扩增法,利用PCR成功地合成了人生长激素基因的全序列。经克隆测序,证明已成功地实现了人生长素基因的合成及克隆。Abstract： According to the amino acids and by selecting high-usage codons in E. coli, we designed and synthesized 20 oligonucleitides DNA fragments with the length about 55bp. By overlap extension PCR method, we successfully synthesized the overall sequence of human growth hormone. The clones of interest were identified by DNA sequencing. The result showed that we have successfully accomplished the synthesis and cloning of human growth hormone.

关 键 词：人生长素基因 基因合成 聚合酶链反应 

分 类 号：Q78[生物学—分子生物学]