视黄酸受体基因在小儿淋巴结的表达与B细胞的发育  

THE EXPRESSION OF RETINOIC ACID RECEPTOR GENES IN LYMPH NODES OF YOUNG CHILDREN AND THE DEVELOPMENT OF B CELLS

作　　者：魏东[1] 杨毅[1] 王卫平[1] 

机构地区：[1]复旦大学附属儿科医院,上海200032

出　　处：《营养学报》2005年第6期460-463,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.0070661)

摘　　要：目的:研究视黄酸受体基因在小儿淋巴结的表达与B细胞发育的关系,阐明视黄酸促进抗体产生的途径与机制。方法:取5岁以下儿童正常淋巴结作冰冻切片,用地高辛素标记六种视黄酸受体基因(RARα、β、γ、RXRα、β、γ)反义RNA探针作原位杂交,及采用RT-荧光定量PCR,观察视黄酸受体基因在淋巴结的表达与分布,分析其与B细胞发育的关系。结果:原位杂交结果显示:六种视黄酸受体基因在淋巴结中的淋巴细胞和网状细胞中均有表达,分布广泛。RT-荧光定量PCR显示:在不同年龄儿童淋巴结六种视黄酸受体基因表达水平有所差异,1岁以下儿童受体基因表达水平普遍较低,此后随免疫发育而升高。结论:视黄酸受体基因的表达可能参与了B细胞的个体发育和免疫应答。这可能是维生素A增强儿童抗感染免疫力的重要环节和介导视黄酸作用的主要途径。Objective： To investigate the influence of the expression of retinoic acid receptor genes on the development of B cells in lymph nodes of young children. Method： In situ hybridization was performed on the frozen section of lymph node of young children （≤age 5 ）. Six digoxin labeled antisense RNA probes for retinoic acid receptors mRNA （ RARα, β, RXRα, β, γ） were used. The expression and distribution of retinoic acid receptor genes in lymph nodes and their influence on the differentiation and maturation of B cell were observed. RT-fluorescent PCR was also used to observe the expression levels of retinoic acid receptor genes and their relation to the maturation of B cells. Results： In situ hybridization showed all the 6 retinoic acid receptor genes were expressed in lymphocytes and reticular cells of lymph node, and the distribution was widespread. RT fluorescent PCR also showed a varying expression of 6 retinoic acid receptor genes in lymph node among different age children, but lower in children younger than 1 year, and then increased gradually with the development of immune system. Conclusion： The expression and regulation of retinoic acid receptor genes may take part in the ontogenesis of B cells, and play a key role in the regulation of retinoic acid and enforce the anti-infective immunity in children.

关 键 词：视黄酸 视黄酸受体 儿童 淋巴结 B淋巴细胞 

分 类 号：R723[医药卫生—儿科]