氯化锂对染铅大鼠海马nNOS蛋白与基因表达的影响  

EFFECT OF LITHIUM ON HIPPOCAMPUS NEURO NITRIC OXIDE SYNTHASE PROTEIN AND GENE EXPRESSION OF LEAD-EXPOSED RATS

作　　者：杨芳[1] 李积胜[1] 

机构地区：[1]武警医学院军事预防医学研究所,天津300162

出　　处：《营养学报》2005年第6期475-478,共4页Acta Nutrimenta Sinica

基　　金：国家自然基金资助(No.39870689)

摘　　要：目的:探讨锂对醋酸铅神经毒性的拮抗效应及其可能机制。方法:通过ABC免疫组化法和半定量逆转录-聚合酶链式反应(RT-PCR)方法,研究饮用0.2g/L醋酸铅(PbAc)饮水和含不同剂量(3、30、300和3000mg/kg)氯化锂(LiCl)饲料喂养的大鼠海马nNOS蛋白与基因表达的变化。结果:与对照组比,3、30mg/kgLiCl组大鼠海马CA1区nNOS阳性神经元数目和nNOSmRNA相对含量显著增多;而染铅组大鼠则显著减少。Pb+LiCl(3、30和300mg/kg)组均较染铅组有显著性增加,但Pb+3000mg/kgLiCl组与染铅组无显著性差异。各组大鼠海马齿状回nNOS阳性神经元数目的变化与CA1区变化基本一致,CA3区变化不显著。结论:锂对铅的神经毒性有明显的拮抗作用。Objective： To investigate the antagonistic effect of lithium on the neurotoxicity of lead acetate and the possible mechanisms. Method： Wistar rats were randomly divided into control group, Pb group and four Pb＋ LiCI groups fed with feed containing 3, 30, 300, 3 000 mg/kg LiCI respectively. All Pb exposed groups were given distilled water containing 0.2% PbAc. The changes of nNOS protein and gene expression in rats hippocampus were studied by ABC immunohistochemistry and the semiquantitative reverse transcription-polymerase chain reaction （RT-PCR） method. Rusults： Compared with the control group, the number of nNOS positive neurons in CA1 area and the nNOS mRNA content of hippocampus of LiCI （3, 30 mg/kg） groups were increased, while those of Pb-exposed rats were significantly decreased, Compared with Pb groups, the number of nNOS positive neurons and the nNOS mRNA content of Pb＋LiCI （3, 30, 300 mg/kg） groups were increased. In dentate gyrus, the changes of the number of nNOS positive neurons coincided with the changes in CAI area, but those in CA3 area showed little difference. Conclusion： Low dose of lithium could resist the lead neurotoxcity obviously.

关 键 词：锂 铅 海马 神经元型一氧化氮合酶 

分 类 号：R114[医药卫生—卫生毒理学]