淋巴细胞共刺激信号和凋亡信号异常与RA免疫效应亢进的研究  

Research of abnormal costimulatory and apoptotic signal to lymphocytes in patients with rheumatoid arthritis

作　　者：王兰兰[1] 蔡蓓[1] 陈捷[1] 冯伟华[1] 

机构地区：[1]四川大学华西医院实验医学科临床免疫实验室,成都610041

出　　处：《中国免疫学杂志》2005年第12期940-943,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(30470683);四川省科技应用基础项目基金(02SY029-126)资助

摘　　要：目的探究T淋巴细胞表面多种细胞信号分子所介导的细胞活化或凋亡信号在RA患者免疫功能紊乱中的作用。方法采用流式细胞术检测RA患者外周血T细胞亚群及其表面共刺激分子CD154(CD40L)、CD30和凋亡受体CD95(Fas)的表达。结果RA患者外周血T细胞亚群偏移,CD4+T细胞增加,CD8+T细胞减少;共刺激分子CD154在CD4+和CD8+T细胞上的表达均上调,但CD30分子的表达均降低,并以CD4+T细胞降低更为明显。同时,凋亡受体CD95分子在T细胞亚群上的表达均明显增加。结论RA患者T淋巴细胞表面多种信号分子表达异常,共同导致了RA患者免疫功能紊乱。Objective：To explore the functions of diversity of signal molecules expressed on T cells inducing activations of cells and apoptosis in causing imbalaneed immunity of patients with RA. Methods：To apply flow eytometer to determine the expression of CD 154（CIMOL）, CD30 and CD95（Fas） on T lymphocytes.Results：Imbalanced T subsets, increasing CD4^＋ T cells and decreasing CD8^＋ T cells were found in peripheral blood of patients with RA. Expressions of CD154 on both CD4^＋ T cells and CD8^＋ T cells increased, but that of CD30, particularly CD4^＋ CD30^＋ T cells decrease. However, expressions of CD95 on T subsets strikingly increased. Conclusion： Abnormal expressions of diverse signal molecules on T lymphocytes lead to the imbalanced immune function in patients with rheumatoid arthritis.

关 键 词：CD154/CD40L CD30 CD95/Fas 类风湿性关节炎 

分 类 号：R392.12[医药卫生—免疫学]