母鼠低剂量甲基汞染毒致亲、仔代肝脏的氧化损伤  

Effects of Maternal Exposure to Low-level Methylmercury on the Growth of Offspring and Lipid Peroxidation in Liver

作　　者：王丽 贾光[1] 闫蕾[1] 王翔[1] 陈春英[2] 高峰 张程程[1] 

机构地区：[1]北京大学医学部公共卫生学院劳动与环境卫生学系 [2]中国科学院高能物理研究所,北京100049 [3]内蒙古包头市昆区防疫站防疫科,014010

出　　处：《环境与职业医学》2005年第6期495-497,518,共4页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金十五重大项目(编号:10490180)

摘　　要：[目的]ICR母鼠通过饮水染毒低剂量氯化甲基汞(MeHgCl),观察MeHgCl对子代生长发育以及亲、仔代氧化损伤的影响。[方法]ICR孕鼠随机分为对照组、低剂量组和高剂量组,各组于怀孕第6天起分别自由饮用蒸馏水和MeHgCl含量分别为0.01、0.1mg/L(相当于饮用水汞含量标准的8倍和80倍剂量)的蒸馏水直至哺乳期结束。研究指标包括母鼠孕期和仔鼠出生后的体重增长及母鼠、仔鼠肝脏中汞含量、脂质过氧化产物丙二醛(MDA)含量及总抗氧化活力(T-AOC)的水平。[结果]在0.01和0.1mg/L剂量下,亲仔两代体重增长各剂量组无差异,也未出现明显的毒性反应,但均有明显的体内汞蓄积。表现为对照组、低剂量组和高剂量组母鼠肝脏汞含量分别为3.703、11.516、100.821ng/g组织湿重,仔鼠肝脏汞含量分别为2.664、5.454、38.846ng/g组织湿重。对照组、低剂量组和高剂量组母鼠总抗氧化活力和MDA含量分别为2.833、2.517、1.974U/mg蛋白和0.849、1.883、2.219nmol/mg蛋白,与对照组相比,甲基汞可使母鼠肝脏总抗氧化活力降低,肝脏中MDA含量增加(P<0.05);对照组、低剂量组和高剂量组仔鼠总抗氧化活力和MDA含量分别为1.860、2.361、2.945U/mg蛋白和0.506、0.702、0.617nmol/mg蛋白,甲基汞使仔鼠总抗氧化活力升高(P<0.05),使低剂量组MDA含量明显高于对照组(P<0.05),但MDA含量高剂量组和对照组相比变化不显著(P>0.05)。[结论]在相当于饮用水汞卫生标准8倍和80倍剂量水平上,低剂量甲基汞虽未引起仔代明显发育障碍,但是可通过母体在仔代体内蓄积,并对亲、仔代肝脏产生不同的氧化损伤。[ Objective ] In order to study the potential effect of low-level methylmercury on the growth of offspring and lipid peroxidation, ICR mice were treated with low-level methylmercury in drinking water. [ Methods ] The pregnant ICR mice were randomly divided into three groups （ control, low-level and high-level groups ）, and were exposed to 0 mg/L, 0.01 mg/L and 0.1 mg/L of methylmercury in drinking water from day 6 of gestation to postnatal day 21.The level of Hg, MDA and T-AOC in the liver of maternal and their offsprings were determined. [ Results ] Without the observed development defects, high level of mercury was detected in the liver of maternal and their offsprings, and the pathological changes of the maternal liver were observed. With the dose increasing, the contents of Hg in liver increased. The level of Hg in maternal mice was higher than in the offsprings. Compared with the controls, the experimental dose of methylmercury can reduce the total ability of antioxidantion, and increase the level of MDA in the liver of maternal mice（ P 〈 0.05 ）;For the offsprings, the production of MDA in low dose group was significantly higher than that of controls, however, no significant difference was observed in the high dose group. [ Conclusion ] The accumulation of mercury can be detected in the livers of offsprings by maternal exposure even to the low dose of methylmercury, and impaired the ability of antioxidantion and lipid peroxidation of maternal and offsprings in a different manner.

关 键 词：甲基汞 生长发育 总抗氧化活力 丙二醛 

分 类 号：R114[医药卫生—卫生毒理学]