工作场所空气中戊二醛测定方法的研究  

A Study on the Determination Method of Glutaral in Workplace

作　　者：张志虎[1] 邵华[1] 

机构地区：[1]山东省职业卫生与职业病防治研究院,济南250062

出　　处：《环境与职业医学》2005年第6期509-510,共2页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金(编号:30471429);山东省自然科学基金(编号:Y2002C30)

摘　　要：[目的]建立一种实用的工作场所空气中戊二醛的采样、测定方法.[方法]用浸渍2,4-二硝基苯肼(DNPH)的硅胶管采样,用乙腈在超声中洗脱,高效液相色谱分离,二极管阵列检测器检测.[结果]方法简便、准确、灵敏度高.标准曲线的相关系数0.998 15,加标回收率99.47%～104.86%,相对标准偏差0.17%～0.20%,平均采样效率100%.[结论]本方法各项指标均达到<工作场所空气中毒物检测方法的研制规范>的要求,能准确地测定空气中戊二醛.[ Objective ] To establish an useful method for sampling and detecting glutaral in the workplace. [ Methods ] Glass tubes filled with DNPH-coated silica gel were used for air sampling.The DNPH-derivatives were extracted from the silica gel by ultrasonic treatment with acetonitrile.Analysis of the DNPH-derivatives was carried out using a HPLC with diodenarray （ UV/DAD ）detector. [ Results ] The method was simple, accurate, and highly sensitive.The correlation coefficient of calibration curves was 0.998.Recovery rates were 99.47-104.86%.The relative standard deviations were 0.17-0.20% and average sampling efficiency was about 100%. [ Conclusion ] This method conforms with the requirements of “Standardization of Methods for Determination of Toxic Substance in Workplace” .Glutaral in air could be determined accurately using this method.

关 键 词：戊二醛 检测 高效液相色谱法 工作场所 

分 类 号：R134.4[医药卫生—劳动卫生]