藻蓝蛋白对脑缺血再灌注后NF-κB和IL-6表达及神经细胞凋亡的影响  

Effects of phycocyanin on the expressions of NF-κB,IL-6 and neuronal apoptosis after cerebral ischemic reperfusion in rats

作　　者：张冬梅[1] 刘吉东[2] 陈红兵[1] 段德麟[2] 郭云良[1] 

机构地区：[1]青岛大学医学院附属医院脑血管病研究所,山东青岛266003 [2]中国科学院海洋研究所海洋生物学开放实验室,山东青岛266071

出　　处：《中国海洋药物》2005年第6期6-10,共5页Chinese Journal of Marine Drugs

基　　金：国家973重点基础研究发展规划资助项目(G1999012004)

摘　　要：目的研究藻蓝蛋白对局灶性脑缺血再灌注大鼠脑保护作用的可能机制。方法大鼠60只,采用改良线栓法建立大脑中动脉闭塞再灌注(MCAO/R)模型,应用藻蓝蛋白进行治疗,观察脑缺血再灌注后神经细胞核因子-κB,IL-6免疫表达和神经细胞凋亡变化,以及藻蓝蛋白对上述指标的影响。结果脑缺血再灌注6h开始,皮质区和纹状体区神经细胞NF-кB表达逐渐增强,于再灌注1d达高峰,之后逐渐下降。IL-6表达部位和变化规律与NF-кB相似,但其表达高峰在再灌注2d。神经细胞凋亡于脑缺血再灌注6h开始逐渐增加,在皮质区和纹状体区分别与再灌注1d和2d达到高峰,其变化规律与NF-кB和IL-6表达一致。应用藻蓝蛋白后,神经细胞NF-κB和IL-6表达显著减低,神经元凋亡明显减少。结论藻蓝蛋白具有抑制脑缺血再灌注后神经细胞凋亡、下调NF-κB和IL-6表达的作用。Objective To investigate the protective effect of phycocyanin on focal cerebral ischemia reperfusion in rats. Methods The model of the middle cerebral artery occlusion and reperfusion （MCAO/R） was established using the intraluminal filament occlusion with 60 healthy adult male Wistar rats, and treated by phycocyanin. The expressions of NF-κB, IL-6 and neuronal apoptosis were detected to evaluate the effects of phycocyanin on above indexes. Results The expression level of NF-κB increased successfully from reperfusion 6h, peaked at reperfusion 1d, and then decreased in cortex and striatum in ischemic group. The expression site and change principle of IL-6 were similar to those of NF-κB except of expression peak at reperfusion 2d. The neuronal apoptosis increased successfully from reperfusion 6h, reached peak at reperfusion 1d and 2d in cortex and striatum respectively, and the ehanged charaeter was coincident with NF-κB and IL-6 expressions. The expressions of NF-κB and IL-6 reduced and the number of neuronal apoptosis decreased significantly after applying phycocyanin compared to the control group in the same time. Conclusion Phycocyanin could inhibit neuronal apoptosis and down-regulate the expressions of NF-κB and IL-6 after cerebral ischemia-reperfusion.

关 键 词：藻蓝蛋白 脑缺血 核因子-ΚB 白介素-6 神经细胞凋亡 

分 类 号：R931.711[医药卫生—生药学] R96[医药卫生—药学]