丙型肝炎病毒3′非编码区准种研究  

A study on the quasispecies character of 3′untranlated region in hepatitis C virus

作　　者：秦兆习[1] 张光彩[1] 魏来[2] 丛旭[2] 蒋栋[2] 陈红松[2] 

机构地区：[1]江苏省徐州市中心医院感染科,江苏徐州221009 [2]北京大学肝病研究所

出　　处：《中国医师杂志》2005年第12期1598-1600,共3页Journal of Chinese Physician

基　　金：国家自然科学基金(39770684;30170844);国家"十五"科技攻关计划(2001BA705B06)资助

摘　　要：目的获得中国大陆1b型丙型肝炎病毒(HCV)3′非编码区 (3′UTR)全长序列,探讨在丙型肝炎病毒3′非编码区(高变区)是否存在复杂的准种特性.方法利用逆转录套式聚合酶链反应(RT-PCR)限制性内切酶长度多态性分析(RFLP)初步筛选出6例1b型HCV感染者,采用半套式RT-PCR法扩增出约400 bp的cDNA片段,每例选择12～15个克隆测序.结果每例获得了5～8株全长1b型HCV3′非编码区克隆,由高变区,Poly(u)区,Poly(u/c)区及98碱基区四部分组成;各克隆中序列变异位点主要分布于高变区,Poly(u/c)区,核苷酸同源性0.2%～2.1%,呈现明显的准种分布特征.结论在丙型肝炎病毒3′非编码区(高变区)存在复杂的准种特性.Objective To investigate the the quasispeeies character of 3＇ untranlated region（3＇ UTR） in hepatitis C virus（HCV） by analysing the nueleotide sequence polymorphism and mutation features in 3＇ UTR region, Methods Patients infected with genotype lb HCV were identified by reverse transcription -nested polymerase chain reaction （lit- PCR） and restriction fragment length polymorphism （RFLP） assay, Fragments of the eDNA of 3＇ UTR were amplified using semi -nested lit -PCR, and subjected to cloning. The 12 - 15 clones that contained HCV 3＇UTR gene fragments amplified from each patients were sequenced. Results The full - length sequence of l b genotype HCV 3＇UTR in eDNA were obtained. The 3＇UTR region consists of four elements ： the 5＇ region, the poly （U） , poly （U/C） and 98 - base region. The nueleotide sequence diversity ranged from 0. 2% - 2. 1% and the mutation points were almost distributed in the the 5 ＇region and poly（U/C）. Conclusions The HCV has complex quasispeeies character in 3＇ UTR.

关 键 词：丙型肝炎病毒 3’非编码区 准种 逆转录套式聚合酶链反应 核苷酸 

分 类 号：R373[医药卫生—病原生物学]