盐藻中β—胡萝卜素的分离与测定──纸层析法  

SEPARATION AND DETERMINATION OF β-CAROTENE IN DUNALLIENA SALINA──PAPER CHROMATOGRAPHY

作　　者：胡慧玲[1] 罗金生[1] 

机构地区：[1]新疆工学院化学工程系,乌鲁木齐830008

出　　处：《新疆工学院学报》1996年第2期121-124,共4页

摘　　要：盐藻中含有多种营养物质，其中蛋白质，甘油，叶绿素及β—胡萝卜素具有开发价值．利用β—胡萝卜素极性最小，易溶于石油醚的特点，用丙酮一石油醚提取盐藻中的β—胡萝卜素，纸层析分离，分光光度法于450um波长处测定，可定量测定出盐藻中的β—胡萝卜素的含量．本文利用此法对多个盐藻样品进行了测定，变异系数为7．4％，回收率在84．1％—112．0％之间．Dunalliena salina is a kind of matter, in which many nutritien substances can be found. Its β-caro-tene and protein et al have been exploited by scientists. Having the least polarity and good solubility in petrol-mether, carotene can be abstracted completely from dunelliena salina with acetone and petrolenether. And it can be separated by paper chromatography and determined by spectrophotometry.

关 键 词：胡萝卜素 盐藻 分离 纸层析法 

分 类 号：Q949.2[生物学—植物学]