消增颗粒淫羊藿苷的测定(英文)  

Determination of icariin in Xiaozeng granules

作　　者：王京霞[1] 许家鸾[1] 

机构地区：[1]浙江省中医院,浙江杭州310006

出　　处：《中国现代应用药学》2005年第6期489-491,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立高效液相色谱法测定消增颗粒中淫羊藿苷的含量方法.方法采用HP1100高效液相色谱仪(包括G1314A可变波长紫外检测器,HP化学工作站,G1322A真空脱气机,HP1100四元泵),Hypersil ODS柱(4.6mm×250mm,5μm).流动相:乙腈-水(30:70),流速:1.0mL·min-1,检测波长:270 nm,进样量:20μl.结果淫羊藿苷在0.16～4.0μg范围内呈现良好的线性关系,回收率为98.6%(n=5),日内平均RSD为1.7%,日间平均RSD为2.1%.结论该法简便、准确、具有专属性,可用于测定消增颗粒淫羊藿苷的含量.OBJECTIVE To establish an HPLC method for analysis of ieariin in XIAO ZENG granules. METHODS The HPLC system consisted of G1314A variable wavelength uv-visible detector, Hypersil ODS （4.6ram × 250mm, 5μm）, HP chemstation, Hp1100 series quaternary pump and vacuum dagasser （G1322A）. The mobile phase was acetanitrile：water （30： 70, v/v）. The flowrate was 1.0mL min^- 1,the injection volume was 20μl, and the detector wavelength was 270nm. RESULTS The method was linear over the iearlin range of 0.16μg to 4.0μg. the recovery of method was 98.6% （ n = 5 ）, the average RSD was 1.7% for precision of within-day, the average RSD was 2.1% for precision of betwean-day. CONCLUSION The method is simple, accurate and selective, can be used for determination of icariin.

关 键 词：消增颗粒 淫羊藿苷 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]