百合皂苷的提取、纯化及其对自由基的清除作用  

Extraction of Saponins from Lilium brownii and Its Scavenging Effect on Hydroxyl Radical

作　　者：吴晓斌[1] 任凤莲[1] 邱昌桂[1] 李彤[1] 吴泓毅[1] 

机构地区：[1]中南大学化学化工学院,长沙410083

出　　处：《天然产物研究与开发》2005年第6期777-780,共4页Natural Product Research and Development

摘　　要：本文用正交实验法对百合总皂甙的提取工艺中温度、乙醇浓度、固液比例、回流时间和提取次数5个因素进行研究,优选出简便可靠、且适合工业化生产的百合总皂甙的提取工艺。其最佳提取工艺条件是:温度为60℃,乙醇浓度为70%,固液比例为1∶6,提取时间3h,提取次数3次。以溴邻苯三酚红(BPR)为显色剂,基于Co(II)H2O2体系反应产生的羟自由基(·OH)使溴邻苯三酚红(BPR)的颜色发生变化,采用756型紫外可见分光光度计测定其吸光度的变化值,研究百合皂苷在此体系中清除羟自由基的作用,对照实验表明:百合总皂苷提取物对羟自由基的清除作用比人参皂苷强。Based on orthogonal design, five factors in extraction of saponins from Lilium brownii were optimized： concentration of ethanol, temperature, solld/liquid ratio, reflux time and extraction times. The optimized process for extracting saponins from Lilium brownii is confirmed as follows： temperature, 60℃; alcohol, 70%; solld/liquid, 1 ： 6; reflux time, 3 h; extraction, 3 times. The radical scavenging activity of saponins is studied. By comparing the effects of saponins from ginseng saponins, the result shows that Lilium brownii saponins have a good scavenging effect on hydroxyl radical.

关 键 词：百合 总皂甙 提取 自由基 清除作用 

分 类 号：O629.13[理学—有机化学]