电磁脉冲辐射对小鼠睾丸血-睾屏障的影响  

Radiation effects of electromagnetic pulses on mouse blood-testis barrier

作　　者：侯武刚[1] 赵洁[1] 张远强[1] 曾丽华[2] 郭国桢[2] 

机构地区：[1]第四军医大学基础部组织学与胚胎学教研室,西安710032 [2]第四军医大学预防医学系放射医学教研室,西安710032

出　　处：《辐射研究与辐射工艺学报》2005年第6期366-370,共5页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(30370750)全军医药卫生科研基金(01MA183)资助

摘　　要：采用常规苏木精-伊红染色(Hematoxylin-Eosin staining,HE staining)、硝酸镧示踪电镜和埃文思兰 (Evans Blue,EB)尾静脉注射三种方法,综合观察100kV／m和400kV／m两种不同强度电磁脉冲辐射对小 鼠睾丸血-睾屏障的影响。三种方法观察结果基本一致,两种强度的电磁脉冲辐射均可造成小鼠睾丸支持细胞和 血一睾屏障不同程度的损伤,400 kV/m辐射组损伤更甚,辐射后1 d即可见大量生精细胞的凋亡与坏死,支持细 胞亦发生形态结构的变化,血-睾屏障严重损伤,随后大量第Ⅷ期生精小管内出现基底室与近腔室的分离,大量 凋亡与坏死的生精细胞向管腔的排放,至辐射后21d仍可见到血-睾屏障损伤;100kV/m辐射组的损伤较 400 kV/m辐射组稍轻,辐射后14 d支持细胞、生精上皮以及血-睾屏障的损伤已基本恢复。结果提示一定强度 的电磁脉冲可以造成小鼠血-睾屏障的损伤,这种损伤呈辐射强度依赖性,并且与损伤后恢复时间相适应。Radiation effects caused by 100kV/m and 400kV/m electromagnetic pulse （EMP） irradiations on mouse blood-testis barrier were studied by means of routine HE staining, Lanthanum traced electron microscope and injection of caudal vein with Evans Blue. The EMP irradiation of different dose rates damaged Sertoli＇s cell and blood-testis barrier of mouse testis in different levels. Severe injuries were observed with the 400 kV/m irradiation group, with apoptosis and necrosis in a large quantity of the spermatogenic cells, shape and structural changes of the Sertoli＇s cells, and serious injuries to the blood-testis barrier, one day after the irradiation. The basal compartment separated from the adluminal compartment in most of the Ⅷ stage seminiferous epithelium, and a great number of apoptosis and necrosis spermatogenic cells were released into the cavities. Injuries of blood-testis barrier could be observed 21 days after the 400 kV/m irradiation. The injuries of 100 kV/m irradiation groups were less severe than the 400 kV/m groups, in which the damages to the Sertoli＇s cells, the seminiferous epithelium and blood-testis barrier recovered to some extent 14 days after the irradiation. We conclude that EMP irradiation can damage mouse blood-testis barrier. The injuries, and the time for recovery, are related to EMP power intensity.

关 键 词：电磁脉冲辐射 血-睾屏障 支持细胞 埃文思兰 硝酸镧 睾丸 

分 类 号：R818[医药卫生—放射医学] Q492.4[医药卫生—临床医学]