RGD多肽修饰的改性PLGA仿生支架材料对骨髓间充质干细胞粘附性影响的研究  

MSC adhesion to PLGA modified with RGD peptide

作　　者：李长文[1] 郑启新[1] 郭晓东[1] 全大萍[2] 赵洁[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院骨科,武汉430022 [2]中山大学高分子研究所,广州510275

出　　处：《华中医学杂志》2005年第6期439-441,共3页Central China Medical Journal

基　　金：国家自然科学基金资助项目(No.30170270)

摘　　要：目的了解精氨酸甘氨酸天冬氨酸(RGD多肽)修饰是否能提高改性的PLGA仿生支架材料对骨髓间充质干细胞的粘附性。方法用异型双功能交联剂SulfoLCSPDP将GRGDSPC多肽共价结合到改性PLGA支架材料上,以未接多肽的改性PLGA材料做对照,取第三代MSC接种到材料上,培养4、12小时后沉淀法定量检测粘附的细胞数,培养24小时后摄光镜图像比较粘附细胞的数量和形态。结果培养4、12小时后实验组细胞粘附率分别为0.17±0.01、0.38±0.02,均极显著高于对照组的0.08±0.01、0.14±0.01(P<0.01),且24小时后细胞的粘附质量也较对照组为好。结论RGD多肽修饰的确能提高改性PLGA支架材料对骨髓间充质干细胞的粘附性。Objective To study the effect of prepared PLGA modified with RGD peptide on the adhesion of MSC to this biomaterials.Methods Heterobifunctional cross-linker, Sulfo-LC-SPDP was used immobilize GRGDSPC on the surface of PLGA. The third generation MSCs were seeded onto the biomaterials and incubated for 4 h and 12 h at standard condition. At each point the number of adhesive cells was counted to evaluate the efficiency of cell adhesion, and the photo of adhesive cells cultured for 24 h was taken to evaluate the quality of cell adhesion.Results It was found at 4 h and 12 h the efficiency of cell adhesion in the modified PLGA was higher than in unmodified PLGA, and at 24 h the quality of cell adhesion in modified PLGA was better.Conclusion MSCs adhesion to PLGA can be enhanced by modifying the PLGA with RGD peptide.

关 键 词：细胞粘附 生物材料 表面修饰 RGD多肽 PLGA 

分 类 号：R318.08[医药卫生—生物医学工程]