大鼠气管-支气管上皮细胞的分离、鉴定和培养  

A Method for Isolating、Identifying and Culturing of Rat Trachea-bronchia Epithelial Cells.

作　　者：崔凤梅[1] 苏世标[1] 聂继华[1] 李冰燕[1] 童建[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院,江苏苏州215007

出　　处：《中国辐射卫生》2005年第4期246-247,共2页Chinese Journal of Radiological Health

摘　　要：目的探讨大鼠气管-支气管上皮细胞的分离、鉴定和培养的方法。方法采用链酶蛋白酶消化联合细胞刷刷洗气管-支气管分离上皮细胞,激光共聚焦显微镜下鉴定,于无血清完全F12培养基中培养。结果实验中可得到大约106个细胞/鼠,并且有较高的成活率和细胞纯度,在无血清完全F12培养基中细胞生长良好。结论消化后刷洗法是一种实用的大鼠气管-支气管上皮细胞提取方法,无血清完全F12培养基是一种可行的培养体系。Objective To explore a method for isolating,identifying and culturing the rat trachea-bronchia epithelial cells. Methods The rat trachea-bronchia epithelial cells were isolated by digestion with pmnase and brushing with cell brush, identified using confocul and cultured in entire F12 media with no serum. Results With this method, cells in high purity and high viability could be obtained,and about 106 cells per rat. The cells grow well in entire F12 media with no serum. Conclusion The method is useful for isolating rat trachea-branchia epithelial cells and the entire F12 media with no serum is effective for culturing.

关 键 词：气管-支气管上皮细胞 分离 鉴定 培养 

分 类 号：R967[医药卫生—药理学]