乌头碱直流电离子导入对大鼠关节炎的镇痛作用及其机制探讨  

The mechanism underlying the analgesic effect of the iontophoresis of aconitine on the adjuvant arthritis in rat

作　　者：康乐[1] 岳寿伟[1] 王晓聆[1] 方明[1] 

机构地区：[1]山东大学齐鲁医院康复医学科,济南250012

出　　处：《中华物理医学与康复杂志》2005年第12期733-736,共4页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的探讨乌头碱直流电离子导入对大鼠关节炎的镇痛机制。方法Wistar大鼠45只,随机分为空白对照组(空白组,n=15)、致炎模型组(模型组,n=15)和乌头碱直流电离子导入治疗组(治疗组,n=15),每组又根据观察时间点分为1d、5d和10d亚组,每亚组5只。制作大鼠佐剂性关节炎(AA)模型后,治疗组行乌头碱直流电离子导入治疗,于实验第1,5和10天分别取3组大鼠下丘脑、脑干组织及局部炎性组织,采用放射免疫法测定大鼠下丘脑β-内啡肽(β-EP)含量,采用高效液相色谱法测定大鼠脑干5-羟色胺(5-HT)、去甲肾上腺素(NE)及局部炎性组织5-HT含量。结果模型组大鼠各时间点踝关节炎性组织5-HT的含量明显高于空白组(P<0.01);治疗组乌头碱导入治疗1d后,局部炎性组织5-HT含量与模型组比较,差异无统计学意义(P>0.05),治疗5d及10d后,5-HT含量明显低于模型组(P<0.05)。模型组大鼠各时间点脑干5-HT含量均明显低于空白组,下丘脑β-EP明显高于空白组(P<0.05);治疗组治疗5d及10d后,脑干5-HT和下丘脑β-EP含量均明显高于模型组(P<0.05)。模型组大鼠各时间点脑干NE含量与空白组比较,差异无统计学意义(P>0.05);治疗组治疗5d及10d后,脑干NE含量明显高于模型组(P<0.05)。结论多次乌头碱直流电离子导入治疗能降低炎性组织局部5-HT含量,升高脑干5-HT、NE和下丘脑β-EP的含量,产生镇痛作用。Objective To study the mechanism of analgesic effects of the iontophoresis of aconitine on the rat with adjuvant arthritis. Methods Forty-five Wistar rats were randomly divided into a normal control group, a model group and a therapy group, with 15 rats in each group. The model of rat adjuvant arthritis（AA） was induced by injection of complete Freud＇s adjuvant（0.1 ml） into the right ankle joint of the rats. The brain stem, hypothalami and local inflammation tissue of rats in the 3 groups were obtained after 1, 5 and 10 days of treatment, respectively. The concentrations of 5-HT, NE, β-EP in brain stem, hypothalami and local inflammation tissue were detected with high performance liquid chromatography （HPLC） and radioimmunoassay （RIA）. Results It was shown that the 5-HT content in the inflammatory tissues was not significantly different from that in the model group after 1 day of treat-ment, but significantly lower than that in the model group after 5 to 10 days of treatment （ P 〈 0.05 ）. Compared with the normal control group, the content of brain stem 5-HT was significantly lower, and that of hypothalami β-EP was significantly higher in the model group （ P 〈 0. 05 ）. After 5 to 10 days of treatment, the brain stem 5-HT and hypothalami β-EP in the therapy group were significantly higher than those in the model group. There was no signifi- cant difference between the model and the normal control groups with regard to brain stem NE content （ P 〉 0.05 ）. After 5 to 10 days of treatment, the brain stem NE content in the therapy group was significantly higher than that in the model group（P 〈 0.05 ）. Conclusion Iontophoresis of aconitine can change the content of 5-HT, NE, β-EP in central nervous system and 5-HT in inflammatatory tissues of the AA rats.

关 键 词：乌头碱 离子导入 关节炎 5-羟色胺 去甲肾上腺素 Β-内啡肽 

分 类 号：R285[医药卫生—中药学]